In vitro effects of eicosanoids derived from different 20-carbon fatty acids on production of monocyte-derived cytokines in human whole blood cultures
In vitro effects of eicosanoids derived from different 20-carbon fatty acids on production of monocyte-derived cytokines in human whole blood cultures
Introduction: Prostaglandins (PG) and leukotrienes (LT) are usually formed from arachidonic acid (e.g. PGE2, LTB4, LTC4). The anti-inflammatory effects of fish oil may be mediated through the production of alternative PG and/or LT formed from eicosapentaenoic acid (e.g. PGE3, LTC5). This study examines the effects of PG and LT derived from different fatty acid precursors on lipopolysaccharide-induced cytokine production by cultured human whole blood.
Methods: Human whole blood was diluted 1:5 and incubated for 48 h with lipopolysaccharide. PGE and LT were added and the concentrations of inflammatory cytokines in the cell culture supernatants determined.
Results: Tumour necrosis factor (TNF)-? concentrations were significantly decreased by the addition of PGE. At the maximum concentration used (10?6 M) TNF-? concentration was reduced to 100%, 90% and 70% by PGE1, PGE2 and PGE3 respectively. Likewise, interleukin (IL)-1? concentration was decreased to 60%, 30% and 40% by 10?6 M PGE1, PGE2 and PGE3, respectively. IL-6 and IL-10 concentrations were not altered by PG. LTB4, LTC4 or LTC5 did not significantly affect cytokine concentrations.
Conclusions: PGE inhibit lipopolysaccharide-stimulated TNF-? and IL-1? production in human whole blood cultures. PGE1, PGE2 and PGE3 show a similar pattern and magnitude of effect. This suggests that the anti-inflammatory effects of dietary fish oil may not be mediated through a simple substitution of one family of eicosanoids for another.
cytokine, eicosanoid, prostaglandin, leukotriene, interleukin
215-223
Miles, E.A.
20332899-ecdb-4214-95bc-922dde36d416
Allen, E.
6c0cddd9-80f5-4864-87ff-5fb08f0e8a1c
Calder, P.C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
2002
Miles, E.A.
20332899-ecdb-4214-95bc-922dde36d416
Allen, E.
6c0cddd9-80f5-4864-87ff-5fb08f0e8a1c
Calder, P.C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Miles, E.A., Allen, E. and Calder, P.C.
(2002)
In vitro effects of eicosanoids derived from different 20-carbon fatty acids on production of monocyte-derived cytokines in human whole blood cultures.
Cytokine, 20 (5), .
(doi:10.1006/cyto.2002.2007).
Abstract
Introduction: Prostaglandins (PG) and leukotrienes (LT) are usually formed from arachidonic acid (e.g. PGE2, LTB4, LTC4). The anti-inflammatory effects of fish oil may be mediated through the production of alternative PG and/or LT formed from eicosapentaenoic acid (e.g. PGE3, LTC5). This study examines the effects of PG and LT derived from different fatty acid precursors on lipopolysaccharide-induced cytokine production by cultured human whole blood.
Methods: Human whole blood was diluted 1:5 and incubated for 48 h with lipopolysaccharide. PGE and LT were added and the concentrations of inflammatory cytokines in the cell culture supernatants determined.
Results: Tumour necrosis factor (TNF)-? concentrations were significantly decreased by the addition of PGE. At the maximum concentration used (10?6 M) TNF-? concentration was reduced to 100%, 90% and 70% by PGE1, PGE2 and PGE3 respectively. Likewise, interleukin (IL)-1? concentration was decreased to 60%, 30% and 40% by 10?6 M PGE1, PGE2 and PGE3, respectively. IL-6 and IL-10 concentrations were not altered by PG. LTB4, LTC4 or LTC5 did not significantly affect cytokine concentrations.
Conclusions: PGE inhibit lipopolysaccharide-stimulated TNF-? and IL-1? production in human whole blood cultures. PGE1, PGE2 and PGE3 show a similar pattern and magnitude of effect. This suggests that the anti-inflammatory effects of dietary fish oil may not be mediated through a simple substitution of one family of eicosanoids for another.
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Published date: 2002
Keywords:
cytokine, eicosanoid, prostaglandin, leukotriene, interleukin
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Local EPrints ID: 25809
URI: http://eprints.soton.ac.uk/id/eprint/25809
ISSN: 1043-4666
PURE UUID: 0f5dc238-ef7e-46a7-9dae-329e2ea1c7b2
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Date deposited: 19 Apr 2006
Last modified: 16 Mar 2024 02:51
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Author:
E. Allen
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