Production of lymphocyte-derived cytokines by whole umbilical cord blood cultures stimulated with mitogens and allergens
Production of lymphocyte-derived cytokines by whole umbilical cord blood cultures stimulated with mitogens and allergens
A whole blood culture technique was used to establish conditions for stimulating the production of cytokines by cord blood lymphocytes. The cultures were stimulated with mitogens (concanavalin A and phytohaemagglutinin) and allergens (beta-lactoglobulin (BLG), ovalbumin (OVA) and house dust mite (Der p 1)) at a range of concentrations. Interleukin (IL-) 2, IL-4, IL-10, IL-13 and interferon-gamma (IFN-?) concentrations were assayed in the supernatants at 24, 48 and 72 h. Stimulation with mitogens but not allergens induced increases in IL-2 and IL-13 concentrations. IFN-? was strongly induced by mitogenic stimulation: maximal responses were seen at 48 h. Stimulation with the allergens also induced an increase in IFN-? concentration which was maximal for 100 ?g/ml of BLG and OVA. Der p 1 induced the highest IFN-? production among the allergens. IL-4 concentrations were increased in mitogen and Der p 1 stimulated cultures. This was maximal at 48 and 24 h, respectively. IL-10 was induced with mitogen and allergen stimulation. Thus, this study has established conditions for assessing production of lymphocyte-derived cytokines in a simple whole umbilical cord blood culture system.
allergen, cord blood, cytokine, interleukin, whole blood
74-83
Miles, Elizabeth A.
20332899-ecdb-4214-95bc-922dde36d416
Bakewell, Lucy
a987b19b-8281-4c9e-bc6e-1358844f8509
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
2003
Miles, Elizabeth A.
20332899-ecdb-4214-95bc-922dde36d416
Bakewell, Lucy
a987b19b-8281-4c9e-bc6e-1358844f8509
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Miles, Elizabeth A., Bakewell, Lucy and Calder, Philip C.
(2003)
Production of lymphocyte-derived cytokines by whole umbilical cord blood cultures stimulated with mitogens and allergens.
Cytokine, 21 (2), .
(doi:10.1016/S1043-4666(02)00502-1).
Abstract
A whole blood culture technique was used to establish conditions for stimulating the production of cytokines by cord blood lymphocytes. The cultures were stimulated with mitogens (concanavalin A and phytohaemagglutinin) and allergens (beta-lactoglobulin (BLG), ovalbumin (OVA) and house dust mite (Der p 1)) at a range of concentrations. Interleukin (IL-) 2, IL-4, IL-10, IL-13 and interferon-gamma (IFN-?) concentrations were assayed in the supernatants at 24, 48 and 72 h. Stimulation with mitogens but not allergens induced increases in IL-2 and IL-13 concentrations. IFN-? was strongly induced by mitogenic stimulation: maximal responses were seen at 48 h. Stimulation with the allergens also induced an increase in IFN-? concentration which was maximal for 100 ?g/ml of BLG and OVA. Der p 1 induced the highest IFN-? production among the allergens. IL-4 concentrations were increased in mitogen and Der p 1 stimulated cultures. This was maximal at 48 and 24 h, respectively. IL-10 was induced with mitogen and allergen stimulation. Thus, this study has established conditions for assessing production of lymphocyte-derived cytokines in a simple whole umbilical cord blood culture system.
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Published date: 2003
Keywords:
allergen, cord blood, cytokine, interleukin, whole blood
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Local EPrints ID: 25811
URI: http://eprints.soton.ac.uk/id/eprint/25811
ISSN: 1043-4666
PURE UUID: 56ea6e7d-187b-48d8-a904-a66d97b7d2df
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Date deposited: 20 Apr 2006
Last modified: 16 Mar 2024 02:51
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Author:
Lucy Bakewell
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