The utilisation of electromagnetic fields in DNA microchip electrophoresis
The utilisation of electromagnetic fields in DNA microchip electrophoresis
A numerical model is presented for the accurate and efficient prediction of pre-concentration and transport of DNA in micro-capillary electrophoresis. The model, which is based on the Finite Element-Flux Corrected Transport (FE-FCT) method, incorporates conservation laws for the different buffer ions, salt ions and DNA sample, coupled through the solution of Poisson’s equation to account for the field modifications that cause electro-migration. The model has been employed for the prediction of DNA sample pre-concentration and transport during electrophoresis in a double-T injector micro-device and to test its validity, the numerical results have been compared with the corresponding experimental data under similar conditions with excellent agreement obtained. The results demonstrate the great potential offered by the model for future optimisation of such in terms of enhanced speed and resolution of separation.
dna, electrophoresis, microchip
85-86
Srivastava, A.
8e5ac80a-3d45-4271-aed4-e4bd122fe9f6
Georghiou, G. E.
27c937e2-8024-4c7e-86be-5656ef10cf64
Metaxas, A. C.
7c183e7d-f2d4-4618-8223-79077a7e7de7
Matsudaira, P.
c94d9bf4-e00a-445a-b3e0-ee4eba08d61d
Ehrlich, D.
d79bc60b-b35e-4adf-8b02-c6c68516a974
2004
Srivastava, A.
8e5ac80a-3d45-4271-aed4-e4bd122fe9f6
Georghiou, G. E.
27c937e2-8024-4c7e-86be-5656ef10cf64
Metaxas, A. C.
7c183e7d-f2d4-4618-8223-79077a7e7de7
Matsudaira, P.
c94d9bf4-e00a-445a-b3e0-ee4eba08d61d
Ehrlich, D.
d79bc60b-b35e-4adf-8b02-c6c68516a974
Srivastava, A., Georghiou, G. E., Metaxas, A. C., Matsudaira, P. and Ehrlich, D.
(2004)
The utilisation of electromagnetic fields in DNA microchip electrophoresis.
Fifth IEE International Conference on Computation in Electromagnetics, Stratford-upon-Avon.
18 - 21 Apr 2004.
.
Record type:
Conference or Workshop Item
(Paper)
Abstract
A numerical model is presented for the accurate and efficient prediction of pre-concentration and transport of DNA in micro-capillary electrophoresis. The model, which is based on the Finite Element-Flux Corrected Transport (FE-FCT) method, incorporates conservation laws for the different buffer ions, salt ions and DNA sample, coupled through the solution of Poisson’s equation to account for the field modifications that cause electro-migration. The model has been employed for the prediction of DNA sample pre-concentration and transport during electrophoresis in a double-T injector micro-device and to test its validity, the numerical results have been compared with the corresponding experimental data under similar conditions with excellent agreement obtained. The results demonstrate the great potential offered by the model for future optimisation of such in terms of enhanced speed and resolution of separation.
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Published date: 2004
Additional Information:
Event Dates: 19-22 April 2004
Venue - Dates:
Fifth IEE International Conference on Computation in Electromagnetics, Stratford-upon-Avon, 2004-04-18 - 2004-04-21
Keywords:
dna, electrophoresis, microchip
Organisations:
Electronics & Computer Science
Identifiers
Local EPrints ID: 259310
URI: http://eprints.soton.ac.uk/id/eprint/259310
PURE UUID: 7ee5de2e-f45b-4701-9480-3e885277ee46
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Date deposited: 04 May 2004
Last modified: 27 Apr 2022 12:05
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Contributors
Author:
A. Srivastava
Author:
G. E. Georghiou
Author:
A. C. Metaxas
Author:
P. Matsudaira
Author:
D. Ehrlich
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