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The utilisation of electromagnetic fields in DNA microchip electrophoresis

The utilisation of electromagnetic fields in DNA microchip electrophoresis
The utilisation of electromagnetic fields in DNA microchip electrophoresis
A numerical model is presented for the accurate and efficient prediction of pre-concentration and transport of DNA in micro-capillary electrophoresis. The model, which is based on the Finite Element-Flux Corrected Transport (FE-FCT) method, incorporates conservation laws for the different buffer ions, salt ions and DNA sample, coupled through the solution of Poisson’s equation to account for the field modifications that cause electro-migration. The model has been employed for the prediction of DNA sample pre-concentration and transport during electrophoresis in a double-T injector micro-device and to test its validity, the numerical results have been compared with the corresponding experimental data under similar conditions with excellent agreement obtained. The results demonstrate the great potential offered by the model for future optimisation of such in terms of enhanced speed and resolution of separation.
dna, electrophoresis, microchip
85-86
Srivastava, A.
8e5ac80a-3d45-4271-aed4-e4bd122fe9f6
Georghiou, G. E.
27c937e2-8024-4c7e-86be-5656ef10cf64
Metaxas, A. C.
7c183e7d-f2d4-4618-8223-79077a7e7de7
Matsudaira, P.
c94d9bf4-e00a-445a-b3e0-ee4eba08d61d
Ehrlich, D.
d79bc60b-b35e-4adf-8b02-c6c68516a974
Srivastava, A.
8e5ac80a-3d45-4271-aed4-e4bd122fe9f6
Georghiou, G. E.
27c937e2-8024-4c7e-86be-5656ef10cf64
Metaxas, A. C.
7c183e7d-f2d4-4618-8223-79077a7e7de7
Matsudaira, P.
c94d9bf4-e00a-445a-b3e0-ee4eba08d61d
Ehrlich, D.
d79bc60b-b35e-4adf-8b02-c6c68516a974

Srivastava, A., Georghiou, G. E., Metaxas, A. C., Matsudaira, P. and Ehrlich, D. (2004) The utilisation of electromagnetic fields in DNA microchip electrophoresis. Fifth IEE International Conference on Computation in Electromagnetics, Stratford-upon-Avon. 18 - 21 Apr 2004. pp. 85-86 .

Record type: Conference or Workshop Item (Paper)

Abstract

A numerical model is presented for the accurate and efficient prediction of pre-concentration and transport of DNA in micro-capillary electrophoresis. The model, which is based on the Finite Element-Flux Corrected Transport (FE-FCT) method, incorporates conservation laws for the different buffer ions, salt ions and DNA sample, coupled through the solution of Poisson’s equation to account for the field modifications that cause electro-migration. The model has been employed for the prediction of DNA sample pre-concentration and transport during electrophoresis in a double-T injector micro-device and to test its validity, the numerical results have been compared with the corresponding experimental data under similar conditions with excellent agreement obtained. The results demonstrate the great potential offered by the model for future optimisation of such in terms of enhanced speed and resolution of separation.

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More information

Published date: 2004
Additional Information: Event Dates: 19-22 April 2004
Venue - Dates: Fifth IEE International Conference on Computation in Electromagnetics, Stratford-upon-Avon, 2004-04-18 - 2004-04-21
Keywords: dna, electrophoresis, microchip
Organisations: Electronics & Computer Science

Identifiers

Local EPrints ID: 259310
URI: http://eprints.soton.ac.uk/id/eprint/259310
PURE UUID: 7ee5de2e-f45b-4701-9480-3e885277ee46

Catalogue record

Date deposited: 04 May 2004
Last modified: 01 Oct 2020 16:34

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