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Contribution of JAM-1 to epithelial differentiation and tight-junction biogenesis in the mouse preimplantation embryo

Record type: Article

We have investigated the contribution of the tight junction (TJ) transmembrane protein junction-adhesion-molecule 1 (JAM-1) to trophectoderm epithelial differentiation in the mouse embryo. JAM-1-encoding mRNA is expressed early from the embryonic genome and is detectable as protein from the eight-cell stage. Immunofluorescence confocal analysis of staged embryos and synchronized cell clusters revealed JAM-1 recruitment to cell contact sites occurred predominantly during the first hour after division to the eight-cell stage, earlier than any other TJ protein analysed to date in this model and before E-cadherin adhesion and cell polarization. During embryo compaction later in the fourth cell cycle, JAM-1 localized transiently yet precisely to the apical microvillous pole, where protein kinase C (PKC) and PKC are also found, indicating a role in cell surface reorganization and polarization. Subsequently, in morulae and blastocysts, JAM-1 is distributed ubiquitously at cell contact sites within the embryo but is concentrated within the trophectoderm apicolateral junctional complex, a pattern resembling that of E-cadherin and nectin-2. However, treatment of embryos with anti-JAM-1-neutralizing antibodies indicated that JAM-1 did not contribute to global embryo compaction and adhesion but rather regulated the timing of blastocoel cavity formation dependent upon establishment of the trophectoderm TJ paracellular seal.

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Citation

Thomas, F.C., Sheth, B., Eckert, J.J., Bazzoni, G., Dejana, E. and Fleming, T.P. (2004) Contribution of JAM-1 to epithelial differentiation and tight-junction biogenesis in the mouse preimplantation embryo Journal of Cell Science, 117, (23), pp. 5599-5608. (doi:10.1242/jcs.01424).

More information

Published date: 2004
Additional Information: All the work for this paper took place within my laboratory and funded by my grants (Tom Fleming)
Keywords: junction adhesion molecule, tight junction, trophectoderm epithelium, cell polarity, blastocyst, Adherens junction

Identifiers

Local EPrints ID: 26030
URI: http://eprints.soton.ac.uk/id/eprint/26030
ISSN: 0021-9533
PURE UUID: db0f4582-2019-4432-bd55-318b8d83e2c9

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Date deposited: 12 Apr 2006
Last modified: 17 Jul 2017 16:08

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Contributors

Author: F.C. Thomas
Author: B. Sheth
Author: J.J. Eckert
Author: G. Bazzoni
Author: E. Dejana
Author: T.P. Fleming

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