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Contribution of JAM-1 to epithelial differentiation and tight-junction biogenesis in the mouse preimplantation embryo

Contribution of JAM-1 to epithelial differentiation and tight-junction biogenesis in the mouse preimplantation embryo
Contribution of JAM-1 to epithelial differentiation and tight-junction biogenesis in the mouse preimplantation embryo
We have investigated the contribution of the tight junction (TJ) transmembrane protein junction-adhesion-molecule 1 (JAM-1) to trophectoderm epithelial differentiation in the mouse embryo. JAM-1-encoding mRNA is expressed early from the embryonic genome and is detectable as protein from the eight-cell stage. Immunofluorescence confocal analysis of staged embryos and synchronized cell clusters revealed JAM-1 recruitment to cell contact sites occurred predominantly during the first hour after division to the eight-cell stage, earlier than any other TJ protein analysed to date in this model and before E-cadherin adhesion and cell polarization. During embryo compaction later in the fourth cell cycle, JAM-1 localized transiently yet precisely to the apical microvillous pole, where protein kinase C (PKC) and PKC are also found, indicating a role in cell surface reorganization and polarization. Subsequently, in morulae and blastocysts, JAM-1 is distributed ubiquitously at cell contact sites within the embryo but is concentrated within the trophectoderm apicolateral junctional complex, a pattern resembling that of E-cadherin and nectin-2. However, treatment of embryos with anti-JAM-1-neutralizing antibodies indicated that JAM-1 did not contribute to global embryo compaction and adhesion but rather regulated the timing of blastocoel cavity formation dependent upon establishment of the trophectoderm TJ paracellular seal.
junction adhesion molecule, tight junction, trophectoderm epithelium, cell polarity, blastocyst, Adherens junction
0021-9533
5599-5608
Thomas, F.C.
83929fc6-362c-4908-8214-9a42861c3b43
Sheth, B.
2ca6ed58-a992-47b7-b3a5-3c5df82aada7
Eckert, J.J.
729bfa49-7053-458d-8e84-3e70e4d98e57
Bazzoni, G.
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Dejana, E.
ecb1de45-fd9e-435b-8187-23b66511d7d5
Fleming, T.P.
2abf761a-e5a1-4fa7-a2c8-12e32d5d4c03
Thomas, F.C.
83929fc6-362c-4908-8214-9a42861c3b43
Sheth, B.
2ca6ed58-a992-47b7-b3a5-3c5df82aada7
Eckert, J.J.
729bfa49-7053-458d-8e84-3e70e4d98e57
Bazzoni, G.
6fc90617-4f09-4e8c-82e7-25418f09e670
Dejana, E.
ecb1de45-fd9e-435b-8187-23b66511d7d5
Fleming, T.P.
2abf761a-e5a1-4fa7-a2c8-12e32d5d4c03

Thomas, F.C., Sheth, B., Eckert, J.J., Bazzoni, G., Dejana, E. and Fleming, T.P. (2004) Contribution of JAM-1 to epithelial differentiation and tight-junction biogenesis in the mouse preimplantation embryo. Journal of Cell Science, 117 (23), 5599-5608. (doi:10.1242/jcs.01424).

Record type: Article

Abstract

We have investigated the contribution of the tight junction (TJ) transmembrane protein junction-adhesion-molecule 1 (JAM-1) to trophectoderm epithelial differentiation in the mouse embryo. JAM-1-encoding mRNA is expressed early from the embryonic genome and is detectable as protein from the eight-cell stage. Immunofluorescence confocal analysis of staged embryos and synchronized cell clusters revealed JAM-1 recruitment to cell contact sites occurred predominantly during the first hour after division to the eight-cell stage, earlier than any other TJ protein analysed to date in this model and before E-cadherin adhesion and cell polarization. During embryo compaction later in the fourth cell cycle, JAM-1 localized transiently yet precisely to the apical microvillous pole, where protein kinase C (PKC) and PKC are also found, indicating a role in cell surface reorganization and polarization. Subsequently, in morulae and blastocysts, JAM-1 is distributed ubiquitously at cell contact sites within the embryo but is concentrated within the trophectoderm apicolateral junctional complex, a pattern resembling that of E-cadherin and nectin-2. However, treatment of embryos with anti-JAM-1-neutralizing antibodies indicated that JAM-1 did not contribute to global embryo compaction and adhesion but rather regulated the timing of blastocoel cavity formation dependent upon establishment of the trophectoderm TJ paracellular seal.

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Published date: 2004
Additional Information: All the work for this paper took place within my laboratory and funded by my grants (Tom Fleming)
Keywords: junction adhesion molecule, tight junction, trophectoderm epithelium, cell polarity, blastocyst, Adherens junction

Identifiers

Local EPrints ID: 26030
URI: http://eprints.soton.ac.uk/id/eprint/26030
ISSN: 0021-9533
PURE UUID: db0f4582-2019-4432-bd55-318b8d83e2c9

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Date deposited: 12 Apr 2006
Last modified: 08 Jan 2022 12:54

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Contributors

Author: F.C. Thomas
Author: B. Sheth
Author: J.J. Eckert
Author: G. Bazzoni
Author: E. Dejana
Author: T.P. Fleming

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