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Prostaglandin E2 production and T cell function after fish-oil supplementation: response to antioxidant cosupplementation

Prostaglandin E2 production and T cell function after fish-oil supplementation: response to antioxidant cosupplementation
Prostaglandin E2 production and T cell function after fish-oil supplementation: response to antioxidant cosupplementation
Background: prostaglandin E2 (PGE2) inhibits lymphocyte proliferation and the production of interferon-{gamma} (IFN-{gamma}) by peripheral blood mononuclear cells, but the effect of PGE2 on interleukin 4 (IL-4) production is unclear. Fish oil, which contains eicosapentaenoic and docosahexaenoic acids, inhibits production of PGE2. The effects of fish oil on lymphocyte proliferation and production of IFN-{gamma} and IL-4 are unclear and may be influenced by the availability of antioxidants.
Objective: we investigated the effect of dietary fish oil with and without antioxidant cosupplementation on lymphocyte proliferation and the production of PGE2, IFN-{gamma}, and IL-4 by peripheral blood mononuclear cells.
Design: sixteen healthy men received dietary fish-oil supplements providing 0.3, 1, and 2 g eicosapentaenoic acid plus docosahexaenoic acid/d for 4 consecutive weeks each (total of 12 wk). All subjects were randomly assigned to daily cosupplementation with either antioxidants (200 µg Se, 3 mg Mn, 30 mg RRR-{alpha}-tocopheryl succinate, 90 mg ascorbic acid, 450 µg vitamin A) or placebo.
Results: fish-oil supplementation decreased PGE2 production and increased IFN-{gamma} production and lymphocyte proliferation from baseline values. Cosupplementation with antioxidants did not affect cytokine production or lymphocyte proliferation.
Conclusion: dietary fish oil modulates production of IFN-{gamma} and lymphocyte proliferation in a manner consistent with decreased production of PGE2, but this effect is not modified by antioxidant cosupplementation.
fish oil, lymphocyte proliferation, prostaglandin E2, interleukin 4
0002-9165
376-382
Trebble, Timothy M.
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Wootton, Stephen A.
bf47ef35-0b33-4edb-a2b0-ceda5c475c0c
Miles, Elizabeth A.
20332899-ecdb-4214-95bc-922dde36d416
Mullee, Mark
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Arden, Nigel K.
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Ballinger, Anne B.
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Stroud, Mike A.
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Burdge, Graham C.
09d60a07-8ca1-4351-9bf1-de6ffcfb2159
Calder, Phillip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Trebble, Timothy M.
48119242-d090-4778-afd9-58f451dbeecf
Wootton, Stephen A.
bf47ef35-0b33-4edb-a2b0-ceda5c475c0c
Miles, Elizabeth A.
20332899-ecdb-4214-95bc-922dde36d416
Mullee, Mark
fd3f91c3-5e95-4f56-8d73-260824eeb362
Arden, Nigel K.
23af958d-835c-4d79-be54-4bbe4c68077f
Ballinger, Anne B.
719f5da5-3970-4629-8902-3077188d262b
Stroud, Mike A.
1665ae65-0898-4848-bf0d-baec8f2bb078
Burdge, Graham C.
09d60a07-8ca1-4351-9bf1-de6ffcfb2159
Calder, Phillip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6

Trebble, Timothy M., Wootton, Stephen A., Miles, Elizabeth A., Mullee, Mark, Arden, Nigel K., Ballinger, Anne B., Stroud, Mike A., Burdge, Graham C. and Calder, Phillip C. (2003) Prostaglandin E2 production and T cell function after fish-oil supplementation: response to antioxidant cosupplementation. American Journal of Clinical Nutrition, 78 (3), 376-382.

Record type: Article

Abstract

Background: prostaglandin E2 (PGE2) inhibits lymphocyte proliferation and the production of interferon-{gamma} (IFN-{gamma}) by peripheral blood mononuclear cells, but the effect of PGE2 on interleukin 4 (IL-4) production is unclear. Fish oil, which contains eicosapentaenoic and docosahexaenoic acids, inhibits production of PGE2. The effects of fish oil on lymphocyte proliferation and production of IFN-{gamma} and IL-4 are unclear and may be influenced by the availability of antioxidants.
Objective: we investigated the effect of dietary fish oil with and without antioxidant cosupplementation on lymphocyte proliferation and the production of PGE2, IFN-{gamma}, and IL-4 by peripheral blood mononuclear cells.
Design: sixteen healthy men received dietary fish-oil supplements providing 0.3, 1, and 2 g eicosapentaenoic acid plus docosahexaenoic acid/d for 4 consecutive weeks each (total of 12 wk). All subjects were randomly assigned to daily cosupplementation with either antioxidants (200 µg Se, 3 mg Mn, 30 mg RRR-{alpha}-tocopheryl succinate, 90 mg ascorbic acid, 450 µg vitamin A) or placebo.
Results: fish-oil supplementation decreased PGE2 production and increased IFN-{gamma} production and lymphocyte proliferation from baseline values. Cosupplementation with antioxidants did not affect cytokine production or lymphocyte proliferation.
Conclusion: dietary fish oil modulates production of IFN-{gamma} and lymphocyte proliferation in a manner consistent with decreased production of PGE2, but this effect is not modified by antioxidant cosupplementation.

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Published date: September 2003
Keywords: fish oil, lymphocyte proliferation, prostaglandin E2, interleukin 4

Identifiers

Local EPrints ID: 26042
URI: http://eprints.soton.ac.uk/id/eprint/26042
ISSN: 0002-9165
PURE UUID: e3c5ceb9-9996-4b18-b888-e9d10291327f
ORCID for Elizabeth A. Miles: ORCID iD orcid.org/0000-0002-8643-0655
ORCID for Graham C. Burdge: ORCID iD orcid.org/0000-0002-7665-2967
ORCID for Phillip C. Calder: ORCID iD orcid.org/0000-0002-6038-710X

Catalogue record

Date deposited: 24 Apr 2006
Last modified: 09 Jan 2022 02:49

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Contributors

Author: Timothy M. Trebble
Author: Mark Mullee
Author: Nigel K. Arden
Author: Anne B. Ballinger
Author: Mike A. Stroud

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