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High speed simultaneous optical and impedance analysis of single particles

High speed simultaneous optical and impedance analysis of single particles
High speed simultaneous optical and impedance analysis of single particles
In this work we report on the development of biochips for the rapid analysis of single cells and other particules. We have developed a device that can simultaneously measure the optical and electrical properties of single cells or other micron-scale particules. The micro flow-cytometer chip consists of a planar electrode array onto which a micro-fluidic channel is fabricated from polyimide. The electrodes are used to measure the impedance of single cells flowing through the channel at hundreds per second. The impedance of single particlesis simultaneously measured at typically two separate frequencies (e.g. 0.5MHz and 2MHz) using a lock-in system and high specification instrumentation amplifiers mounted on top of the micro-fluidic chip. The impedance data provides information on the membrane chracteristics of cells and also the size of the particle. In addition a three-wavelength confocal optical system has been developed which is used to simultaneously interrogate the optical properties of particles. The device can detect small numbers of fluorescently labelled rare particles in a sample that has been used for the analysis of blood and suspensions of latex beads.
Dielectrophoresis, AC electrokinetics, Flow Cytometry, Cell sorting, Microfabrication, Microfluidics
263-272
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Holmes, David
eeff86f7-ab4b-4795-9a01-82ce1275e34f
Green, Nicolas G
d9b47269-c426-41fd-a41d-5f4579faa581
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Holmes, David
eeff86f7-ab4b-4795-9a01-82ce1275e34f
Green, Nicolas G
d9b47269-c426-41fd-a41d-5f4579faa581

Morgan, Hywel, Holmes, David and Green, Nicolas G (2005) High speed simultaneous optical and impedance analysis of single particles. Microtechnologies for the New Millennium 2005, Spain. 09 - 11 May 2005. pp. 263-272 .

Record type: Conference or Workshop Item (Paper)

Abstract

In this work we report on the development of biochips for the rapid analysis of single cells and other particules. We have developed a device that can simultaneously measure the optical and electrical properties of single cells or other micron-scale particules. The micro flow-cytometer chip consists of a planar electrode array onto which a micro-fluidic channel is fabricated from polyimide. The electrodes are used to measure the impedance of single cells flowing through the channel at hundreds per second. The impedance of single particlesis simultaneously measured at typically two separate frequencies (e.g. 0.5MHz and 2MHz) using a lock-in system and high specification instrumentation amplifiers mounted on top of the micro-fluidic chip. The impedance data provides information on the membrane chracteristics of cells and also the size of the particle. In addition a three-wavelength confocal optical system has been developed which is used to simultaneously interrogate the optical properties of particles. The device can detect small numbers of fluorescently labelled rare particles in a sample that has been used for the analysis of blood and suspensions of latex beads.

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More information

Published date: 2005
Additional Information: Event Dates: 9-11 May 2005
Venue - Dates: Microtechnologies for the New Millennium 2005, Spain, 2005-05-09 - 2005-05-11
Keywords: Dielectrophoresis, AC electrokinetics, Flow Cytometry, Cell sorting, Microfabrication, Microfluidics
Organisations: Nanoelectronics and Nanotechnology

Identifiers

Local EPrints ID: 261205
URI: https://eprints.soton.ac.uk/id/eprint/261205
PURE UUID: c590ef4f-b09d-4d38-9645-0336144874b2
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676
ORCID for Nicolas G Green: ORCID iD orcid.org/0000-0001-9230-4455

Catalogue record

Date deposited: 08 Sep 2005
Last modified: 20 Jul 2019 00:59

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Contributors

Author: Hywel Morgan ORCID iD
Author: David Holmes
Author: Nicolas G Green ORCID iD

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