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An analysis of the feasibility of short read sequencing

An analysis of the feasibility of short read sequencing
An analysis of the feasibility of short read sequencing
Several methods for ultra high-throughput DNA sequencing are currently under investigation. Many of these methods yield very short blocks of sequence information (reads). Here we report on an analysis showing the level of genome sequencing possible as a function of read length. It is shown that re-sequencing and de novo sequencing of the majority of a bacterial genome is possible with read lengths of 20–30 nt, and that reads of 50 nt can provide reconstructed contigs (a contiguous fragment of sequence data) of 1000 nt and greater that cover 80% of human chromosome 1.
0305-1048
e171
Whiteford, Nava
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Haslam, Niall
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Weber, Gerald
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Prügel-Bennett, Adam
b107a151-1751-4d8b-b8db-2c395ac4e14e
Essex, Jonathan W.
1d11045c-1ed4-4171-8174-8a809dc38379
Roach, Peter L.
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Bradley, Mark
562b9add-34c4-4620-bfa1-c7c83a0f0900
Neylon, Cameron
697f067b-db25-4c41-9618-28f4b74f73aa
Whiteford, Nava
f084a58e-2c9d-496c-b960-77f4cd08a83b
Haslam, Niall
d4023afb-8897-4649-a6ea-996c3a9402cb
Weber, Gerald
7cfc4eb7-a658-44fd-97e3-b3be79a6615f
Prügel-Bennett, Adam
b107a151-1751-4d8b-b8db-2c395ac4e14e
Essex, Jonathan W.
1d11045c-1ed4-4171-8174-8a809dc38379
Roach, Peter L.
50f9fc91-e333-4be6-9634-6bb6b4415753
Bradley, Mark
562b9add-34c4-4620-bfa1-c7c83a0f0900
Neylon, Cameron
697f067b-db25-4c41-9618-28f4b74f73aa

Whiteford, Nava, Haslam, Niall, Weber, Gerald, Prügel-Bennett, Adam, Essex, Jonathan W., Roach, Peter L., Bradley, Mark and Neylon, Cameron (2005) An analysis of the feasibility of short read sequencing. Nucleic Acids Research, 33 (19), e171.

Record type: Article

Abstract

Several methods for ultra high-throughput DNA sequencing are currently under investigation. Many of these methods yield very short blocks of sequence information (reads). Here we report on an analysis showing the level of genome sequencing possible as a function of read length. It is shown that re-sequencing and de novo sequencing of the majority of a bacterial genome is possible with read lengths of 20–30 nt, and that reads of 50 nt can provide reconstructed contigs (a contiguous fragment of sequence data) of 1000 nt and greater that cover 80% of human chromosome 1.

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Published date: 2005
Organisations: Southampton Wireless Group

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Local EPrints ID: 261605
URI: https://eprints.soton.ac.uk/id/eprint/261605
ISSN: 0305-1048
PURE UUID: ac6d6117-2416-4a37-8eba-80b741aa2c68

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Date deposited: 28 Nov 2005
Last modified: 15 Nov 2018 17:31

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