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An analysis of the feasibility of short read sequencing

Whiteford, Nava, Haslam, Niall, Weber, Gerald, Prügel-Bennett, Adam, Essex, Jonathan W., Roach, Peter L., Bradley, Mark and Neylon, Cameron (2005) An analysis of the feasibility of short read sequencing Nucleic Acids Research, 33, (19), e171.

Record type: Article


Several methods for ultra high-throughput DNA sequencing are currently under investigation. Many of these methods yield very short blocks of sequence information (reads). Here we report on an analysis showing the level of genome sequencing possible as a function of read length. It is shown that re-sequencing and de novo sequencing of the majority of a bacterial genome is possible with read lengths of 20–30 nt, and that reads of 50 nt can provide reconstructed contigs (a contiguous fragment of sequence data) of 1000 nt and greater that cover 80% of human chromosome 1.

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Published date: 2005
Organisations: Southampton Wireless Group


Local EPrints ID: 261605
ISSN: 0305-1048
PURE UUID: ac6d6117-2416-4a37-8eba-80b741aa2c68

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Date deposited: 28 Nov 2005
Last modified: 18 Jul 2017 09:00

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Author: Nava Whiteford
Author: Niall Haslam
Author: Gerald Weber
Author: Adam Prügel-Bennett
Author: Jonathan W. Essex
Author: Peter L. Roach
Author: Mark Bradley
Author: Cameron Neylon

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