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An aqueous formulation of gamma-linolenic acid with anti-proliferative action on human pancreatic cancer cell lines

An aqueous formulation of gamma-linolenic acid with anti-proliferative action on human pancreatic cancer cell lines
An aqueous formulation of gamma-linolenic acid with anti-proliferative action on human pancreatic cancer cell lines
Essential fatty acids, especially [gamma]-linolenic acid (GLA), have been shown to directly inhibit the growth of cancer cell lines in culture. The aim of this study was to see whether an aqueous formulation of GLA works as well as the lithium-based salt. We evaluated the effect of the 1-deoxy-1-methylamino-D-glucitol salt of GLA (MeGLA) on the growth of two human pancreatic cancer cell lines (Panc-1 and MIA PaCa-2) in vitro, and compared its effects with a previously studied formulation, lithium GLA (LiGLA). The effect of time exposure (2-7 days) and difference in concentration (0-1000 [mu]mol/l) were studied using 96-well culture plates. Cell growth was assessed by MTT assay. Control experiments were performed with meglumine alone in similar concentrations. MeGLA had cytostatic and cytotoxic effects on pancreatic cancer cell lines with 50% growth inhibition at 30-100 [mu]mol/l and cytotoxic effects at 60-250 [mu]mol/l. The degree of growth inhibition increased with time of exposure to MeGLA. The anti-proliferative effects of MeGLA were similar to those previously observed with LiGLA. We conclude that MeGLA has equivalent anti-proliferative activity to LiGLA when tested in vitro against pancreatic cancer cell lines and is therefore a suitable alternative to LiGLA for investigation of the in vivo activity of GLA against pancreatic adenocarcinomas.
0959-4973
157-160
Agombar, A.
b4033887-7430-493c-81aa-11f06c650d1f
Cooper, A. J.
f5416583-ac91-425a-b6ca-0fd566ef159c
Johnson, C. D.
e50aa9cd-8c61-4fe3-a0b3-f51cc3a6c74a
Agombar, A.
b4033887-7430-493c-81aa-11f06c650d1f
Cooper, A. J.
f5416583-ac91-425a-b6ca-0fd566ef159c
Johnson, C. D.
e50aa9cd-8c61-4fe3-a0b3-f51cc3a6c74a

Agombar, A., Cooper, A. J. and Johnson, C. D. (2004) An aqueous formulation of gamma-linolenic acid with anti-proliferative action on human pancreatic cancer cell lines. Anti-Cancer Drugs, 15 (2), 157-160.

Record type: Article

Abstract

Essential fatty acids, especially [gamma]-linolenic acid (GLA), have been shown to directly inhibit the growth of cancer cell lines in culture. The aim of this study was to see whether an aqueous formulation of GLA works as well as the lithium-based salt. We evaluated the effect of the 1-deoxy-1-methylamino-D-glucitol salt of GLA (MeGLA) on the growth of two human pancreatic cancer cell lines (Panc-1 and MIA PaCa-2) in vitro, and compared its effects with a previously studied formulation, lithium GLA (LiGLA). The effect of time exposure (2-7 days) and difference in concentration (0-1000 [mu]mol/l) were studied using 96-well culture plates. Cell growth was assessed by MTT assay. Control experiments were performed with meglumine alone in similar concentrations. MeGLA had cytostatic and cytotoxic effects on pancreatic cancer cell lines with 50% growth inhibition at 30-100 [mu]mol/l and cytotoxic effects at 60-250 [mu]mol/l. The degree of growth inhibition increased with time of exposure to MeGLA. The anti-proliferative effects of MeGLA were similar to those previously observed with LiGLA. We conclude that MeGLA has equivalent anti-proliferative activity to LiGLA when tested in vitro against pancreatic cancer cell lines and is therefore a suitable alternative to LiGLA for investigation of the in vivo activity of GLA against pancreatic adenocarcinomas.

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Published date: February 2004

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Local EPrints ID: 26183
URI: https://eprints.soton.ac.uk/id/eprint/26183
ISSN: 0959-4973
PURE UUID: f34b8a82-b663-40d0-851b-ec39fe77f034

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Date deposited: 20 Apr 2006
Last modified: 17 Jul 2017 16:08

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