The University of Southampton
University of Southampton Institutional Repository

Intratumoral expression of a fusogenic membrane glycoprotein enhances the efficacy of replicating adenovirus therapy

Ahmed, A., Jevremovic, D., Suzuki, K., Kottke, T., Thompson, J., Emery, S., Harrington, K., Bateman, A. and Vile, R. (2003) Intratumoral expression of a fusogenic membrane glycoprotein enhances the efficacy of replicating adenovirus therapy Gene therapy, 10, (19), pp. 1663-1671. (doi:10.1038/sj.gt.3302064).

Record type: Article

Abstract

We describe here a novel strategy to enhance the in vivo efficacy of replicating adenovirus therapy, using coinjection of plasmid DNA encoding a fusogenic viral glycoprotein. The combination of fusogenic membrane glycoprotein (FMG)-induced tumor cell fusion and infection with replicating adenovirus effectively treats even large established tumors at doses of plasmid DNA and virus that alone are ineffective.
Adenoviral infection appears to increase the transduction of the tumor cells to a modest degree thereby boosting the FMG-mediated component of the therapy. Simultaneously, syncytial formation enhances the therapeutic effects of viral infection by increasing spread of adenoviral particles through the tumor cell population and by increasing titer of virus released from the tumor cells. This effect is due probably to release of intracellular viral particles upon tumor cell death and also to increased levels of E1A protein within syncytia, whose increased metabolic rate is associated with enhanced levels of protein expression. Cotransduction of tumor cells with replicating adenovirus and FMG-expressing vectors could either be combined within single replicating vectors or could be used in strategies using separate administration of two components, both at lower doses than required for either therapy alone.

Full text not available from this repository.

More information

Published date: 2003
Keywords: syncytia, conditionally replicating adenoviruses, fusogenic membrane glycoproteins

Identifiers

Local EPrints ID: 26184
URI: http://eprints.soton.ac.uk/id/eprint/26184
ISSN: 0969-7128
PURE UUID: 7fba441f-e816-457b-910a-0d23ecc2c66b

Catalogue record

Date deposited: 20 Apr 2006
Last modified: 17 Jul 2017 16:08

Export record

Altmetrics

Contributors

Author: A. Ahmed
Author: D. Jevremovic
Author: K. Suzuki
Author: T. Kottke
Author: J. Thompson
Author: S. Emery
Author: K. Harrington
Author: A. Bateman
Author: R. Vile

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×