The University of Southampton
University of Southampton Institutional Repository

Antibody modulation of antigen presentation: positive and negative effects on presentation of the tetanus toxin antigen via the murine B cell isoform of FcgammaRII

Antibody modulation of antigen presentation: positive and negative effects on presentation of the tetanus toxin antigen via the murine B cell isoform of FcgammaRII
Antibody modulation of antigen presentation: positive and negative effects on presentation of the tetanus toxin antigen via the murine B cell isoform of FcgammaRII
Tetanus toxin has been a valuable model antigen to study the MHC class II-restricted antigen processing pathway and is also frequently used to provide T helper determinants in vaccine formulations. To date most basic studies on the processing of this antigen have utilized human T and B cell clones. As a first step towards extending studies on this antigen into the murine system we have generated a panel of T cell clones and mAb in H-2b and H-2d mice. We investigated the presentation of tetanus toxin C fragment (TTCF) by the murine B cell lines LB27.4 (H-2dxb), A20 (H-2d) and IIA1.6 (H-2d) and the extent to which this could be modulated by the addition of mAb. One mAb, 10G5, induced strikingly enhanced presentation of T cell determinants located in the N-terminal region of TTCF while other antibodies inhibited presentation of these and other epitopes. The enhancing effects of the 10G5 antibody were blocked by the anti-FcR antibody 2.4G2 and were not observed in the FcR-negative IIA1.6 cell line. Interestingly, both FcRIIB1 and FcRIIB2 isoforms of FcRII were able to restore antibody enhanced presentation in IIA1.6 cells but only if the cytoplasmic tails were intact. These results show that the B cell isoform of FcRII (FcRIIB1) can mediate capture and presentation of some antigen/antibody complexes and might play a role in BCR-independent antigen presentation in vivo.
Fc receptor, antigen processing, tetanus toxin, antibody, mouse
0014-2980
530-540
Antoniou, Antony N.
3d711a3b-f688-46f9-823e-5bb925d10ea8
Watts, Colin
29a9d995-5923-44b9-896b-e618a1ce2824
Antoniou, Antony N.
3d711a3b-f688-46f9-823e-5bb925d10ea8
Watts, Colin
29a9d995-5923-44b9-896b-e618a1ce2824

Antoniou, Antony N. and Watts, Colin (2002) Antibody modulation of antigen presentation: positive and negative effects on presentation of the tetanus toxin antigen via the murine B cell isoform of FcgammaRII. European Journal of Immunology, 32 (2), 530-540. (doi:10.1002/1521-4141(200202)32:2<530::AID-IMMU530>3.0.CO;2-X).

Record type: Article

Abstract

Tetanus toxin has been a valuable model antigen to study the MHC class II-restricted antigen processing pathway and is also frequently used to provide T helper determinants in vaccine formulations. To date most basic studies on the processing of this antigen have utilized human T and B cell clones. As a first step towards extending studies on this antigen into the murine system we have generated a panel of T cell clones and mAb in H-2b and H-2d mice. We investigated the presentation of tetanus toxin C fragment (TTCF) by the murine B cell lines LB27.4 (H-2dxb), A20 (H-2d) and IIA1.6 (H-2d) and the extent to which this could be modulated by the addition of mAb. One mAb, 10G5, induced strikingly enhanced presentation of T cell determinants located in the N-terminal region of TTCF while other antibodies inhibited presentation of these and other epitopes. The enhancing effects of the 10G5 antibody were blocked by the anti-FcR antibody 2.4G2 and were not observed in the FcR-negative IIA1.6 cell line. Interestingly, both FcRIIB1 and FcRIIB2 isoforms of FcRII were able to restore antibody enhanced presentation in IIA1.6 cells but only if the cytoplasmic tails were intact. These results show that the B cell isoform of FcRII (FcRIIB1) can mediate capture and presentation of some antigen/antibody complexes and might play a role in BCR-independent antigen presentation in vivo.

Full text not available from this repository.

More information

Published date: 2002
Keywords: Fc receptor, antigen processing, tetanus toxin, antibody, mouse

Identifiers

Local EPrints ID: 26198
URI: http://eprints.soton.ac.uk/id/eprint/26198
ISSN: 0014-2980
PURE UUID: 6a19c9ea-9bff-4b6e-ba86-298c9100e781

Catalogue record

Date deposited: 20 Apr 2006
Last modified: 15 Jul 2019 19:14

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×