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Transforming growth factor ? receptor 1 polyalanine polymorphism and exon 5 mutation analysis in breast and ovarian cancer

Transforming growth factor ? receptor 1 polyalanine polymorphism and exon 5 mutation analysis in breast and ovarian cancer
Transforming growth factor ? receptor 1 polyalanine polymorphism and exon 5 mutation analysis in breast and ovarian cancer
The inactivation or altered expression of TGF-ß receptors or other components of the TGF-ß signaling pathway are common in many cancer types. A germ-line sequence variant of transforming growth factor-ß receptor-1 (TßR-I), which involves the deletion of three alanines (6A) from a nine-alanine stretch (9A), has impaired mediation of TGF-ß antiproliferative signaling. The TßR-I (6A) variant has been reported to occur at an increased frequency in a variety of cancer types and may represent an important hereditary predisposing factor. We have investigated the possible influence of the TßR-I (6A) allele on cancer risk in a cases-control study of 248 controls; 304 women with ovarian cancer; 98 women with endometriosis; and 355 women with breast cancer occurring under the age of 40 years, bilateral breast cancer, or a family history of breast cancer. The TßR-I (6A) allele was significantly associated with breast cancer (odds ratio, 1.6; 95% confidence interval, 1.1–2.5). There was no significant association of this allele with ovarian cancers as a whole, although stratifying according to their histological subtype revealed a significant association with the endometrioid and clear-cell cancers (odds ratio, 2.1; 95% confidence interval, 1.2–3.6). Recently a recurrent somatic CTCTGG->CTGCGTGG insertion mutation in exon 5 of TßR-I was reported in >30% of ovarian cancers. If verified, this would indicate that inactivation of TßR-I is a key step in the development of ovarian cancer, perhaps second only to the inactivation of TP53. We analyzed 55 ovarian and 33 breast cancers for mutations using both single-stranded conformational polymorphism/heteroduplex analysis and direct sequencing. No somatic mutations in exon 5 of TßR-I were detected in any case. Our study provides additional evidence for an association of the TßR-I (6A) allele with cancer predisposition, but we find no evidence of a mutational hot-spot in exon 5 of TßR-I in either ovarian or breast cancers.
1055-9965
211-214
Baxter, Simon W.
f967c87a-2288-499e-b3ca-c288913050b8
Choong, David Y.H.
cc08344b-077d-46fd-986a-3365becd533a
Eccles, Diana M.
5b59bc73-11c9-4cf0-a9d5-7a8e523eee23
Campbell, Ian G.
681789cb-d416-4722-9ba8-449c7af96673
Baxter, Simon W.
f967c87a-2288-499e-b3ca-c288913050b8
Choong, David Y.H.
cc08344b-077d-46fd-986a-3365becd533a
Eccles, Diana M.
5b59bc73-11c9-4cf0-a9d5-7a8e523eee23
Campbell, Ian G.
681789cb-d416-4722-9ba8-449c7af96673

Baxter, Simon W., Choong, David Y.H., Eccles, Diana M. and Campbell, Ian G. (2002) Transforming growth factor ? receptor 1 polyalanine polymorphism and exon 5 mutation analysis in breast and ovarian cancer. Cancer Epidemiology, Biomarkers & Prevention, 11 (2), 211-214.

Record type: Article

Abstract

The inactivation or altered expression of TGF-ß receptors or other components of the TGF-ß signaling pathway are common in many cancer types. A germ-line sequence variant of transforming growth factor-ß receptor-1 (TßR-I), which involves the deletion of three alanines (6A) from a nine-alanine stretch (9A), has impaired mediation of TGF-ß antiproliferative signaling. The TßR-I (6A) variant has been reported to occur at an increased frequency in a variety of cancer types and may represent an important hereditary predisposing factor. We have investigated the possible influence of the TßR-I (6A) allele on cancer risk in a cases-control study of 248 controls; 304 women with ovarian cancer; 98 women with endometriosis; and 355 women with breast cancer occurring under the age of 40 years, bilateral breast cancer, or a family history of breast cancer. The TßR-I (6A) allele was significantly associated with breast cancer (odds ratio, 1.6; 95% confidence interval, 1.1–2.5). There was no significant association of this allele with ovarian cancers as a whole, although stratifying according to their histological subtype revealed a significant association with the endometrioid and clear-cell cancers (odds ratio, 2.1; 95% confidence interval, 1.2–3.6). Recently a recurrent somatic CTCTGG->CTGCGTGG insertion mutation in exon 5 of TßR-I was reported in >30% of ovarian cancers. If verified, this would indicate that inactivation of TßR-I is a key step in the development of ovarian cancer, perhaps second only to the inactivation of TP53. We analyzed 55 ovarian and 33 breast cancers for mutations using both single-stranded conformational polymorphism/heteroduplex analysis and direct sequencing. No somatic mutations in exon 5 of TßR-I were detected in any case. Our study provides additional evidence for an association of the TßR-I (6A) allele with cancer predisposition, but we find no evidence of a mutational hot-spot in exon 5 of TßR-I in either ovarian or breast cancers.

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Published date: 2002

Identifiers

Local EPrints ID: 26216
URI: http://eprints.soton.ac.uk/id/eprint/26216
ISSN: 1055-9965
PURE UUID: f2e99c2e-3c2c-4904-a45f-3b272eeaf120

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Date deposited: 20 Apr 2006
Last modified: 11 Sep 2017 16:32

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