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Lymphoblastoid cells express HLA-B27 homodimers both intracellularly and at the cell surface following endosomal recycling

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The MHC class I allele HLA-B27 is very strongly associated with development of autoimmune spondyloarthritis, although the disease mechanism remains unknown. Class I molecules classically associate in the endoplasmic reticulum (ER) with ?2-microglobulin (?2m) and antigenic peptides for cell surface expression and presentation to T cells. We have previously shown that HLA-B27 is capable of forming ?2m-free disulfide-bonded homodimers in vitro. Here we show that HLA-B27 forms disulfide-bonded homodimers in vivo by two distinct pathways. HLA-B27 homodimers form in the ER but appear unable to egress to the cell surface in human cells. Cell surface HLA-B27 homodimers are abundantly expressed in a variety of lymphoid cell lines. Experiments with inhibitors indicate that HLA-B27 homodimers can arise from cell-surface heterodimers via an endosome-dependent recycling pathway. HLA-B27 homodimer expression on the cell surface of 721.220 is dependent on the unpaired cysteine67 and is inhibited by restoration of tapasin function or by incubation with peptides that bind strongly to HLA-B27 heterodimers. Cell surface expressed HLA-B27 homodimers are likely to be immunologically reactive ligands for NK family immunoreceptors and, hence, could play a pathogenic role in spondyloarthritis.

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Bird, Lucy A., Peh, Chen Au, Kollnberger, Simon, Elliott, Tim, McMichael, Andrew J. and Bowness, Paul (2003) Lymphoblastoid cells express HLA-B27 homodimers both intracellularly and at the cell surface following endosomal recycling European Journal of Immunology, 33, (3), pp. 748-759. (doi:10.1002/eji.200323678).

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Published date: 2003
Keywords: spondyloarthritis, hla-b27, cysteine 67, mhc class 1 recycling, tapasin


Local EPrints ID: 26219
ISSN: 0014-2980
PURE UUID: e4da3540-c807-4dfb-9a88-d7c482eb02d9
ORCID for Tim Elliott: ORCID iD

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Date deposited: 21 Apr 2006
Last modified: 17 Jul 2017 16:08

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Author: Lucy A. Bird
Author: Chen Au Peh
Author: Simon Kollnberger
Author: Tim Elliott ORCID iD
Author: Andrew J. McMichael
Author: Paul Bowness

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