The University of Southampton
University of Southampton Institutional Repository

Proteomic analysis of chronic lymphocytic leukemia subtypes with mutated or unmutated Ig V(H) genes

Proteomic analysis of chronic lymphocytic leukemia subtypes with mutated or unmutated Ig V(H) genes
Proteomic analysis of chronic lymphocytic leukemia subtypes with mutated or unmutated Ig V(H) genes
Chronic lymphocytic leukemia (CLL) is a common hematopoietic malignant disease with variable outcome. CLL has been divided into distinct groups based on whether somatic hypermutation has occurred in the variable region of the immunoglobulin heavy-chain locus or alternatively if the cells express higher levels of the CD38 protein. We have analyzed the proteome of 12 cases of CLL (six mutated (M-CLL) and six unmutated (UM-CLL) immunoglobulin heavy-chain loci; seven CD38-negative and five CD38-positive) using two-dimensional electrophoresis and mass spectrometry. Statistical evaluation using principal component analysis indicated significant differences in patterns of protein expression between the cases with and without somatic mutation. Specific proteins indicated by principal component analysis as varying between the prognostic groups were characterized using mass spectrometry. The levels of F-actin-capping protein ß subunit, 14-3-3 ß protein, and laminin-binding protein precursor were significantly increased in M-CLL relative to UM-CLL. In addition, primary sequence data from tandem mass spectrometry showed that nucleophosmin was present as several protein spots in M-CLL but was not detected in UM-CLL samples, suggesting that several post-translationally modified forms of nucleophosmin vary between these two sample groups. No specific differences were found between CD38-positive and -negative patient samples using the same approach. The results presented show that proteomic analysis can complement other approaches in identifying proteins that may have potential value in the biological and diagnostic distinction between important clinical subtypes of CLL.
1535-9476
1331-1341
Cochran, Duncan A.E.
6074930e-5e4d-4073-ab04-7b997e3dc0f5
Evans, Caroline A.
30457d30-1fc1-4fe2-943e-3825435ee55d
Blinco, David
3b203b46-69bb-49b7-a15c-9202905294b7
Burthem, John
29b9fa31-a214-4142-9081-6c69e1333d74
Stevenson, Freda K.
ba803747-c0ac-409f-a9c2-b61fde009f8c
Gaskell, Simon J.
52949710-6af1-4d89-880d-362ff734e485
Whetton, Anthony D.
d2e48117-3dfc-4713-82dd-afc85bab739c
Cochran, Duncan A.E.
6074930e-5e4d-4073-ab04-7b997e3dc0f5
Evans, Caroline A.
30457d30-1fc1-4fe2-943e-3825435ee55d
Blinco, David
3b203b46-69bb-49b7-a15c-9202905294b7
Burthem, John
29b9fa31-a214-4142-9081-6c69e1333d74
Stevenson, Freda K.
ba803747-c0ac-409f-a9c2-b61fde009f8c
Gaskell, Simon J.
52949710-6af1-4d89-880d-362ff734e485
Whetton, Anthony D.
d2e48117-3dfc-4713-82dd-afc85bab739c

Cochran, Duncan A.E., Evans, Caroline A., Blinco, David, Burthem, John, Stevenson, Freda K., Gaskell, Simon J. and Whetton, Anthony D. (2003) Proteomic analysis of chronic lymphocytic leukemia subtypes with mutated or unmutated Ig V(H) genes. Molecular & Cellular Proteomics, 2 (12), 1331-1341. (doi:10.1074/mcp.M300055-MCP200).

Record type: Article

Abstract

Chronic lymphocytic leukemia (CLL) is a common hematopoietic malignant disease with variable outcome. CLL has been divided into distinct groups based on whether somatic hypermutation has occurred in the variable region of the immunoglobulin heavy-chain locus or alternatively if the cells express higher levels of the CD38 protein. We have analyzed the proteome of 12 cases of CLL (six mutated (M-CLL) and six unmutated (UM-CLL) immunoglobulin heavy-chain loci; seven CD38-negative and five CD38-positive) using two-dimensional electrophoresis and mass spectrometry. Statistical evaluation using principal component analysis indicated significant differences in patterns of protein expression between the cases with and without somatic mutation. Specific proteins indicated by principal component analysis as varying between the prognostic groups were characterized using mass spectrometry. The levels of F-actin-capping protein ß subunit, 14-3-3 ß protein, and laminin-binding protein precursor were significantly increased in M-CLL relative to UM-CLL. In addition, primary sequence data from tandem mass spectrometry showed that nucleophosmin was present as several protein spots in M-CLL but was not detected in UM-CLL samples, suggesting that several post-translationally modified forms of nucleophosmin vary between these two sample groups. No specific differences were found between CD38-positive and -negative patient samples using the same approach. The results presented show that proteomic analysis can complement other approaches in identifying proteins that may have potential value in the biological and diagnostic distinction between important clinical subtypes of CLL.

Full text not available from this repository.

More information

Published date: 2003

Identifiers

Local EPrints ID: 26251
URI: https://eprints.soton.ac.uk/id/eprint/26251
ISSN: 1535-9476
PURE UUID: a508071d-69c4-42be-8c2a-a4793089898d
ORCID for Freda K. Stevenson: ORCID iD orcid.org/0000-0002-0933-5021

Catalogue record

Date deposited: 21 Apr 2006
Last modified: 20 Jul 2019 01:16

Export record

Altmetrics

Contributors

Author: Duncan A.E. Cochran
Author: Caroline A. Evans
Author: David Blinco
Author: John Burthem
Author: Simon J. Gaskell
Author: Anthony D. Whetton

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×