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A new anti-idiotype antibody capable of binding rituximab on the surface of lymphoma cells

A new anti-idiotype antibody capable of binding rituximab on the surface of lymphoma cells
A new anti-idiotype antibody capable of binding rituximab on the surface of lymphoma cells
The chimeric anti-CD20 monoclonal antibody (mAb), rituximab, is an established part of the management of many non-Hodgkin lymphomas. The in vivo action of rituximab remains elusive, and this partially reflects a lack of highly specific reagents to detect rituximab binding at the cell surface. Here we report a new high-affinity mAb (MB2A4) with fine specificity for the idiotype of rituximab. It is able to detect rituximab in vitro, in the presence of high levels of human immunoglobulin G (IgG), in the serum of patients receiving rituximab therapy, and, surprisingly, when rituximab is bound to CD20 on the cell surface. We propose that the anti–idiotype (Id) binds to rituximab molecules bound univalently at the cell surface, facilitated by the relatively high off-rate of rituximab. This reagent provides new insights into the binding of rituximab at the cell surface and demonstrates a mode of binding that could be exploited for the surface detection of other mAbs with clinical and biologic applications.
0006-4971
2540-2542
Cragg, Mark S.
ec97f80e-f3c8-49b7-a960-20dff648b78c
Bayne, Mike B.
28b1c102-cba6-4f52-a5fe-87e3c7fbefb4
Tutt, Alison L
46ce577b-aea1-412d-84ea-fc4dab794469
French, Ruth R.
a95ea7a1-7aeb-4c20-998e-fde663613fd1
Beers, Stephen
a02548be-3ffd-41ab-9db8-d6e8c3b499a2
Glennie, Martin J.
9f6f0eff-4560-48c2-80cd-0ec116110ded
Illidge, Timothy M.
003bddd7-d778-4c77-bcaa-3b71b364d9a1
Cragg, Mark S.
ec97f80e-f3c8-49b7-a960-20dff648b78c
Bayne, Mike B.
28b1c102-cba6-4f52-a5fe-87e3c7fbefb4
Tutt, Alison L
46ce577b-aea1-412d-84ea-fc4dab794469
French, Ruth R.
a95ea7a1-7aeb-4c20-998e-fde663613fd1
Beers, Stephen
a02548be-3ffd-41ab-9db8-d6e8c3b499a2
Glennie, Martin J.
9f6f0eff-4560-48c2-80cd-0ec116110ded
Illidge, Timothy M.
003bddd7-d778-4c77-bcaa-3b71b364d9a1

Cragg, Mark S., Bayne, Mike B., Tutt, Alison L, French, Ruth R., Beers, Stephen, Glennie, Martin J. and Illidge, Timothy M. (2004) A new anti-idiotype antibody capable of binding rituximab on the surface of lymphoma cells. Blood, 104 (8), 2540-2542. (doi:10.1182/blood-2004-05-1733).

Record type: Article

Abstract

The chimeric anti-CD20 monoclonal antibody (mAb), rituximab, is an established part of the management of many non-Hodgkin lymphomas. The in vivo action of rituximab remains elusive, and this partially reflects a lack of highly specific reagents to detect rituximab binding at the cell surface. Here we report a new high-affinity mAb (MB2A4) with fine specificity for the idiotype of rituximab. It is able to detect rituximab in vitro, in the presence of high levels of human immunoglobulin G (IgG), in the serum of patients receiving rituximab therapy, and, surprisingly, when rituximab is bound to CD20 on the cell surface. We propose that the anti–idiotype (Id) binds to rituximab molecules bound univalently at the cell surface, facilitated by the relatively high off-rate of rituximab. This reagent provides new insights into the binding of rituximab at the cell surface and demonstrates a mode of binding that could be exploited for the surface detection of other mAbs with clinical and biologic applications.

Full text not available from this repository.

More information

Published date: 2004
Additional Information: Neoplasia, brief report

Identifiers

Local EPrints ID: 26260
URI: http://eprints.soton.ac.uk/id/eprint/26260
ISSN: 0006-4971
PURE UUID: f7e54163-a803-43bd-bb18-c69884975a60
ORCID for Mark S. Cragg: ORCID iD orcid.org/0000-0003-2077-089X
ORCID for Stephen Beers: ORCID iD orcid.org/0000-0002-3765-3342

Catalogue record

Date deposited: 21 Apr 2006
Last modified: 18 Feb 2021 16:56

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