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Purification of primary malignant B-cells and immunoblot analysis of bcl-2 family proteins

Purification of primary malignant B-cells and immunoblot analysis of bcl-2 family proteins
Purification of primary malignant B-cells and immunoblot analysis of bcl-2 family proteins
The ability to isolate relatively pure populations of primary normal and malignant lymphocytes has brought studies of lymphoid malignancies to the forefront of cancer research. Apoptosis (programmed cell death) plays a key role in controlling normal B-cell numbers, and resistance to apoptosis contributes to lymphomagenesis and reduces the effectiveness of chemotherapy and radiotherapy. Multiple Bcl-2 family proteins orchestrate key life and death decisions in lymphocytes, and the prototypical family member, Bcl-2, is activated by reciprocal translocation in human lymphoma. Here, we describe an immunomagnetic method to isolate and purify malignant B-cells suitable for in vitro analyses from lymph node biopsies. Methods to analyze the expression of Bcl-2 family proteins by immunoblotting also are described.
bcl-2, apoptosis, lymphoma, purification, immunoblotting
1-59259-936-2
1543-1894
1-14
Humana Press
Dallman, Claire
619359e1-d205-4941-88d1-8d9bfd677a91
Packham, Graham
fdabe56f-2c58-469c-aadf-38878f233394
Dallman, Claire
619359e1-d205-4941-88d1-8d9bfd677a91
Packham, Graham
fdabe56f-2c58-469c-aadf-38878f233394

Dallman, Claire and Packham, Graham (2005) Purification of primary malignant B-cells and immunoblot analysis of bcl-2 family proteins. In, Lymphoma: methods and protocols. (Methods in Molecular Medicine, 115) Humana Press, pp. 1-14.

Record type: Book Section

Abstract

The ability to isolate relatively pure populations of primary normal and malignant lymphocytes has brought studies of lymphoid malignancies to the forefront of cancer research. Apoptosis (programmed cell death) plays a key role in controlling normal B-cell numbers, and resistance to apoptosis contributes to lymphomagenesis and reduces the effectiveness of chemotherapy and radiotherapy. Multiple Bcl-2 family proteins orchestrate key life and death decisions in lymphocytes, and the prototypical family member, Bcl-2, is activated by reciprocal translocation in human lymphoma. Here, we describe an immunomagnetic method to isolate and purify malignant B-cells suitable for in vitro analyses from lymph node biopsies. Methods to analyze the expression of Bcl-2 family proteins by immunoblotting also are described.

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More information

Published date: 2005
Keywords: bcl-2, apoptosis, lymphoma, purification, immunoblotting

Identifiers

Local EPrints ID: 26272
URI: https://eprints.soton.ac.uk/id/eprint/26272
ISBN: 1-59259-936-2
ISSN: 1543-1894
PURE UUID: 073c896b-786c-4554-802e-7badd3ac94ff
ORCID for Graham Packham: ORCID iD orcid.org/0000-0002-9232-5691

Catalogue record

Date deposited: 24 Apr 2006
Last modified: 31 Jan 2019 01:37

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Contributors

Author: Claire Dallman
Author: Graham Packham ORCID iD

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