The University of Southampton
University of Southampton Institutional Repository

Letter to Nature. Viral infection switches non-plasmacytoid dendritic cells into high interferon producers

Letter to Nature. Viral infection switches non-plasmacytoid dendritic cells into high interferon producers
Letter to Nature. Viral infection switches non-plasmacytoid dendritic cells into high interferon producers
Type I interferons (IFN-I) are important cytokines linking innate and adaptive immunity. Plasmacytoid dendritic cells make high levels of IFN-I in response to viral infection and are thought to be the major source of the cytokines in vivo. Here, we show that conventional non-plasmacytoid dendritic cells taken from mice infected with a dendritic-cell-tropic strain of lymphocytic choriomeningitis virus make similarly high levels of IFN-I on subsequent culture. Similarly, non-plasmacytoid dendritic cells secrete high levels of IFN-I in response to double-stranded RNA (dsRNA), a major viral signature, when the latter is introduced into the cytoplasm to mimic direct viral infection. This response is partially dependent on the cytosolic dsRNA-binding enzyme protein kinase R4 and does not require signalling through toll-like receptor (TLR), a surface receptor for dsRNA5. Furthermore, we show that sequestration of dsRNA by viral NS1 explains the inability of conventional dendritic cells to produce IFN-I on infection with influenza. Our results suggest that multiple dendritic cell types, not just plasmacytoid cells, can act as specialized interferon-producing cells in certain viral infections, and reveal the existence of a TLR-independent pathway for dendritic cell activation that can be the target of viral interference.
0028-0836
324-328
Diebold, Sandra S.
f1e39d9e-cfd1-4e43-b66a-4dde63a9d56b
Montoya, Maria
0620e3ca-7e29-4591-a359-a94a3d243399
Unger, Hermann
5a745258-f488-44cb-8da9-ac8c62693ad9
Alexopoulou, Lena
a0717406-c518-4a40-aabc-555df341608c
Roy, Polly
eb7049e7-5c35-4325-8a19-badd4a9fe777
Haswell, Linsey E.
7e2a20d5-8dda-42dd-a956-962ce5446337
Al-Shamkhani, Aymen
0a40b3ce-9d71-4d41-9369-7212f0a84504
Flavell, Richard
bb7ce742-4756-43c8-8639-25e35279451d
Borrow, Persephone
e6eb6b06-b8f9-47bc-af78-d3eb04e8cf9b
Reis e Sousa, Caetano
10599377-1e7b-47ca-bffd-3bc8521acdda
Diebold, Sandra S.
f1e39d9e-cfd1-4e43-b66a-4dde63a9d56b
Montoya, Maria
0620e3ca-7e29-4591-a359-a94a3d243399
Unger, Hermann
5a745258-f488-44cb-8da9-ac8c62693ad9
Alexopoulou, Lena
a0717406-c518-4a40-aabc-555df341608c
Roy, Polly
eb7049e7-5c35-4325-8a19-badd4a9fe777
Haswell, Linsey E.
7e2a20d5-8dda-42dd-a956-962ce5446337
Al-Shamkhani, Aymen
0a40b3ce-9d71-4d41-9369-7212f0a84504
Flavell, Richard
bb7ce742-4756-43c8-8639-25e35279451d
Borrow, Persephone
e6eb6b06-b8f9-47bc-af78-d3eb04e8cf9b
Reis e Sousa, Caetano
10599377-1e7b-47ca-bffd-3bc8521acdda

Diebold, Sandra S., Montoya, Maria, Unger, Hermann, Alexopoulou, Lena, Roy, Polly, Haswell, Linsey E., Al-Shamkhani, Aymen, Flavell, Richard, Borrow, Persephone and Reis e Sousa, Caetano (2003) Letter to Nature. Viral infection switches non-plasmacytoid dendritic cells into high interferon producers. Nature, 424 (6946), 324-328. (doi:10.1038/nature01783).

Record type: Article

Abstract

Type I interferons (IFN-I) are important cytokines linking innate and adaptive immunity. Plasmacytoid dendritic cells make high levels of IFN-I in response to viral infection and are thought to be the major source of the cytokines in vivo. Here, we show that conventional non-plasmacytoid dendritic cells taken from mice infected with a dendritic-cell-tropic strain of lymphocytic choriomeningitis virus make similarly high levels of IFN-I on subsequent culture. Similarly, non-plasmacytoid dendritic cells secrete high levels of IFN-I in response to double-stranded RNA (dsRNA), a major viral signature, when the latter is introduced into the cytoplasm to mimic direct viral infection. This response is partially dependent on the cytosolic dsRNA-binding enzyme protein kinase R4 and does not require signalling through toll-like receptor (TLR), a surface receptor for dsRNA5. Furthermore, we show that sequestration of dsRNA by viral NS1 explains the inability of conventional dendritic cells to produce IFN-I on infection with influenza. Our results suggest that multiple dendritic cell types, not just plasmacytoid cells, can act as specialized interferon-producing cells in certain viral infections, and reveal the existence of a TLR-independent pathway for dendritic cell activation that can be the target of viral interference.

Full text not available from this repository.

More information

Published date: 2003

Identifiers

Local EPrints ID: 26279
URI: https://eprints.soton.ac.uk/id/eprint/26279
ISSN: 0028-0836
PURE UUID: 79d79094-41b2-4f58-9fe5-19656e316b23
ORCID for Aymen Al-Shamkhani: ORCID iD orcid.org/0000-0003-0727-4189

Catalogue record

Date deposited: 24 Apr 2006
Last modified: 31 Jan 2019 01:37

Export record

Altmetrics

Contributors

Author: Sandra S. Diebold
Author: Maria Montoya
Author: Hermann Unger
Author: Lena Alexopoulou
Author: Polly Roy
Author: Linsey E. Haswell
Author: Richard Flavell
Author: Persephone Borrow
Author: Caetano Reis e Sousa

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×