Thermal equivalence of DNA duplexes without calculation of melting temperature
Thermal equivalence of DNA duplexes without calculation of melting temperature
The common key to nearly all processes involving DNA is the hybridization and melting of the double helix: from transmission of genetic information and RNA transcription, to polymerase chain reaction and DNA microarray analysis, DNA mechanical nanodevices and DNA computing. Selecting DNA sequences with similar melting temperatures is essential for many applications in biotechnology. We show that instead of calculating these temperatures, a single parameter can be derived from a statistical-mechanics model that conveniently represents the thermodynamic equivalence of DNA sequences. This parameter is shown to order experimental melting temperatures correctly, is much more readily obtained than the melting temperature, and is easier to handle than the numerous parameters of empirical regression models.
Biological physics: Statistical physics, thermodynamics and nonlinear dynamics
55-59
Weber, Gerald
7cfc4eb7-a658-44fd-97e3-b3be79a6615f
Haslam, Niall
d4023afb-8897-4649-a6ea-996c3a9402cb
Whiteford, Nava
f084a58e-2c9d-496c-b960-77f4cd08a83b
Prügel-Bennett, Adam
b107a151-1751-4d8b-b8db-2c395ac4e14e
Essex, Jonathan
c96f07df-93cc-4467-a075-49f8d6f64ef4
Neylon, Cameron
697f067b-db25-4c41-9618-28f4b74f73aa
January 2006
Weber, Gerald
7cfc4eb7-a658-44fd-97e3-b3be79a6615f
Haslam, Niall
d4023afb-8897-4649-a6ea-996c3a9402cb
Whiteford, Nava
f084a58e-2c9d-496c-b960-77f4cd08a83b
Prügel-Bennett, Adam
b107a151-1751-4d8b-b8db-2c395ac4e14e
Essex, Jonathan
c96f07df-93cc-4467-a075-49f8d6f64ef4
Neylon, Cameron
697f067b-db25-4c41-9618-28f4b74f73aa
Weber, Gerald, Haslam, Niall, Whiteford, Nava, Prügel-Bennett, Adam, Essex, Jonathan and Neylon, Cameron
(2006)
Thermal equivalence of DNA duplexes without calculation of melting temperature.
Nature Physics, 2 (1), .
(doi:10.1038/nphys189).
Abstract
The common key to nearly all processes involving DNA is the hybridization and melting of the double helix: from transmission of genetic information and RNA transcription, to polymerase chain reaction and DNA microarray analysis, DNA mechanical nanodevices and DNA computing. Selecting DNA sequences with similar melting temperatures is essential for many applications in biotechnology. We show that instead of calculating these temperatures, a single parameter can be derived from a statistical-mechanics model that conveniently represents the thermodynamic equivalence of DNA sequences. This parameter is shown to order experimental melting temperatures correctly, is much more readily obtained than the melting temperature, and is easier to handle than the numerous parameters of empirical regression models.
Text
nphys189.pdf
- Version of Record
Restricted to Repository staff only
Request a copy
More information
Published date: January 2006
Keywords:
Biological physics: Statistical physics, thermodynamics and nonlinear dynamics
Organisations:
Southampton Wireless Group
Identifiers
Local EPrints ID: 262888
URI: http://eprints.soton.ac.uk/id/eprint/262888
ISSN: 1745-2473
PURE UUID: f90a7300-6003-4ee9-b379-da89cd62cdff
Catalogue record
Date deposited: 10 Aug 2006
Last modified: 14 Mar 2024 07:20
Export record
Altmetrics
Contributors
Author:
Gerald Weber
Author:
Niall Haslam
Author:
Nava Whiteford
Author:
Adam Prügel-Bennett
Author:
Jonathan Essex
Author:
Cameron Neylon
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics