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Thermal equivalence of DNA duplexes without calculation of melting temperature

Weber, Gerald, Haslam, Niall, Whiteford, Nava, Prügel-Bennett, Adam, Essex, Jonathan and Neylon, Cameron (2006) Thermal equivalence of DNA duplexes without calculation of melting temperature Nature Physics, 2, pp. 55-59.

Record type: Article


The common key to nearly all processes involving DNA is the hybridization and melting of the double helix: from transmission of genetic information and RNA transcription, to polymerase chain reaction and DNA microarray analysis, DNA mechanical nanodevices and DNA computing. Selecting DNA sequences with similar melting temperatures is essential for many applications in biotechnology. We show that instead of calculating these temperatures, a single parameter can be derived from a statistical-mechanics model that conveniently represents the thermodynamic equivalence of DNA sequences. This parameter is shown to order experimental melting temperatures correctly, is much more readily obtained than the melting temperature, and is easier to handle than the numerous parameters of empirical regression models.

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Published date: January 2006
Keywords: Biological physics: Statistical physics, thermodynamics and nonlinear dynamics
Organisations: Southampton Wireless Group


Local EPrints ID: 262888
ISSN: 1745-2473
PURE UUID: f90a7300-6003-4ee9-b379-da89cd62cdff

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Date deposited: 10 Aug 2006
Last modified: 18 Jul 2017 08:47

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Author: Gerald Weber
Author: Niall Haslam
Author: Nava Whiteford
Author: Adam Prügel-Bennett
Author: Jonathan Essex
Author: Cameron Neylon

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