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Nuclear envelope-limited chromatin sheets are part of mitotic death

Nuclear envelope-limited chromatin sheets are part of mitotic death
Nuclear envelope-limited chromatin sheets are part of mitotic death
Nuclear envelope-limited chromatin sheets (ELCS) are enigmatic membranous structures of uncertain function. This study describes the induction of ELCS in p53 mutated Burkitt's lymphoma cell lines after treatment with irradiation or the microtubule inhibitor, SK&F 96365. Both treatments evoked similar mitotic death, involving metaphase arrest followed by extensive endopolyploidisation and delayed apoptosis, although the kinetics were different. We found that induction of ELCS and nuclear segmentation correlated with the amount and kinetics of M-phase arrest, mitosis restitution and delayed apoptosis of endopolyploid cells. In metaphases undergoing restitution, ELCS are seen participating in the restoration of the nuclear envelope, mediating the attachment of peripheral chromatin to it. In interphase cells, ELCS join nuclear segments, ectopically linking and fusing with heterochromatin regions. In cells with segmented nuclei, continued DNA replication was observed, along with activation and redistribution of Ku70, suggestive of non-homologous DNA end-joining. Induction of ELCS also parallels the induction of cytoplasmic stacked membrane structures, such as confronting cisternae and annulate lamellae, which participate in the turnover and degeneration of ELCS. The results suggest that arrest at a spindle checkpoint and the uncoupling of mitosis from DNA replication lead to the emergence of ELCS in the resulting endopolyploid cells.
nuclear envelope-limited chromatin sheets, irradiation, microtubule inhibitors, mitotic arrest, endopolyploidy
0948-6143
243-255
Erenpreisa, Jekaterina
70b5fecb-7208-431f-bd35-ec498edc0033
Ivanov, Andrey
0b43b5f4-c35e-4ee8-9fd6-cffe3bbefb1e
Cragg, Mark
ec97f80e-f3c8-49b7-a960-20dff648b78c
Selivanova, Galina
06b865c5-f5aa-4bf1-b82e-7bacee80dba0
Illidge, Timothy
9abbc831-d66d-4288-8dc4-d2e1ad45d782
Erenpreisa, Jekaterina
70b5fecb-7208-431f-bd35-ec498edc0033
Ivanov, Andrey
0b43b5f4-c35e-4ee8-9fd6-cffe3bbefb1e
Cragg, Mark
ec97f80e-f3c8-49b7-a960-20dff648b78c
Selivanova, Galina
06b865c5-f5aa-4bf1-b82e-7bacee80dba0
Illidge, Timothy
9abbc831-d66d-4288-8dc4-d2e1ad45d782

Erenpreisa, Jekaterina, Ivanov, Andrey, Cragg, Mark, Selivanova, Galina and Illidge, Timothy (2002) Nuclear envelope-limited chromatin sheets are part of mitotic death. Histochemistry and Cell Biology, 117 (3), 243-255. (doi:10.1007/s00418-002-0382-6).

Record type: Article

Abstract

Nuclear envelope-limited chromatin sheets (ELCS) are enigmatic membranous structures of uncertain function. This study describes the induction of ELCS in p53 mutated Burkitt's lymphoma cell lines after treatment with irradiation or the microtubule inhibitor, SK&F 96365. Both treatments evoked similar mitotic death, involving metaphase arrest followed by extensive endopolyploidisation and delayed apoptosis, although the kinetics were different. We found that induction of ELCS and nuclear segmentation correlated with the amount and kinetics of M-phase arrest, mitosis restitution and delayed apoptosis of endopolyploid cells. In metaphases undergoing restitution, ELCS are seen participating in the restoration of the nuclear envelope, mediating the attachment of peripheral chromatin to it. In interphase cells, ELCS join nuclear segments, ectopically linking and fusing with heterochromatin regions. In cells with segmented nuclei, continued DNA replication was observed, along with activation and redistribution of Ku70, suggestive of non-homologous DNA end-joining. Induction of ELCS also parallels the induction of cytoplasmic stacked membrane structures, such as confronting cisternae and annulate lamellae, which participate in the turnover and degeneration of ELCS. The results suggest that arrest at a spindle checkpoint and the uncoupling of mitosis from DNA replication lead to the emergence of ELCS in the resulting endopolyploid cells.

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More information

Published date: 2002
Keywords: nuclear envelope-limited chromatin sheets, irradiation, microtubule inhibitors, mitotic arrest, endopolyploidy

Identifiers

Local EPrints ID: 26296
URI: http://eprints.soton.ac.uk/id/eprint/26296
ISSN: 0948-6143
PURE UUID: 5bdb9bdf-0a0e-440c-8f53-3b64e1f49f70
ORCID for Mark Cragg: ORCID iD orcid.org/0000-0003-2077-089X

Catalogue record

Date deposited: 20 Apr 2006
Last modified: 26 Nov 2019 01:57

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