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Microdevices for Dielectrophoretic Flow-Through Cell Separation

Microdevices for Dielectrophoretic Flow-Through Cell Separation
Microdevices for Dielectrophoretic Flow-Through Cell Separation
A novel dielectrophoretic cell-separation technique has been developed for the separation of white blood cells. A microdevice was constructed from two consecutive arrays of microelectrodes, one to focus the particles into a sheet and the second to separate them using a combination of positive and negative dielectrophoresis. At a flow rate of 1 ml/hour, experiments showed that THP-1 cells were captured on the electrode array. The experimental results were compared with a 2-D numerical simulation of the particle dynamics. The device is shown to be effective at fractionating subpopulations of cells into different bands along the array.
0739-5175
85-90
Holmes, David
eeff86f7-ab4b-4795-9a01-82ce1275e34f
Green, Nicolas G
d9b47269-c426-41fd-a41d-5f4579faa581
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Holmes, David
eeff86f7-ab4b-4795-9a01-82ce1275e34f
Green, Nicolas G
d9b47269-c426-41fd-a41d-5f4579faa581
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Holmes, David, Green, Nicolas G and Morgan, Hywel (2003) Microdevices for Dielectrophoretic Flow-Through Cell Separation. IEEE Engineering in Medicine and Biology Magazine, 22 (6), 85-90. (doi:10.1109/MEMB.2003.1266051).

Record type: Article

Abstract

A novel dielectrophoretic cell-separation technique has been developed for the separation of white blood cells. A microdevice was constructed from two consecutive arrays of microelectrodes, one to focus the particles into a sheet and the second to separate them using a combination of positive and negative dielectrophoresis. At a flow rate of 1 ml/hour, experiments showed that THP-1 cells were captured on the electrode array. The experimental results were compared with a 2-D numerical simulation of the particle dynamics. The device is shown to be effective at fractionating subpopulations of cells into different bands along the array.

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More information

Published date: December 2003
Organisations: Electronics & Computer Science

Identifiers

Local EPrints ID: 263058
URI: https://eprints.soton.ac.uk/id/eprint/263058
ISSN: 0739-5175
PURE UUID: 7bd72f6b-ff0f-43f5-a600-7b620caf1d39
ORCID for Nicolas G Green: ORCID iD orcid.org/0000-0001-9230-4455
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 08 Nov 2006
Last modified: 20 Jul 2019 00:59

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