Dielectrophoresis of DNA Time-and Frequency-Dependent Collections on Microelectrodes
Dielectrophoresis of DNA Time-and Frequency-Dependent Collections on Microelectrodes
This paper reports measurements that characterize the collection of DNA onto interdigitated microelectrodes by high-frequency dielectrophoresis. Measurements of time-dependent collection of 12 kilobase pair plasmid DNA onto microelectrodes by dielectrophoresis show significant reduction in the response as the frequency increases from 100 kHz to 20 MHz. Collection time profiles are quantitatively measured using fluorescence microscopy over the range 100Khz to 5MHz and are represented in terms of two parameters: the initial dielectrophoretic collection rate and the initial to steady-state collection transition. Measured values for both parameters are consistent with trends in the frequency-dependent real part of the effective polarizability measured for the same plasmid DNA using dielectrophoretic spectroscopy. The experimentally measured parameters are qualitatively compared with trends predicted by theory that takes into account dielectrophoretic particle movement and diffusion. The differences between experiment and theory are discussed with suggested improvements to theoretical models, for example, including effects of electrohydronamically driven fluid motion.
AC electrokinetics, dielectrohpretic collection rate, DNA concentration, nonuniform electric fields
Bakewell, David J
2674b57d-b981-46c9-9154-679435db9a94
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
March 2006
Bakewell, David J
2674b57d-b981-46c9-9154-679435db9a94
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Bakewell, David J and Morgan, Hywel
(2006)
Dielectrophoresis of DNA Time-and Frequency-Dependent Collections on Microelectrodes.
IEEE Transactions on Nanobioscience, 5 (1).
Abstract
This paper reports measurements that characterize the collection of DNA onto interdigitated microelectrodes by high-frequency dielectrophoresis. Measurements of time-dependent collection of 12 kilobase pair plasmid DNA onto microelectrodes by dielectrophoresis show significant reduction in the response as the frequency increases from 100 kHz to 20 MHz. Collection time profiles are quantitatively measured using fluorescence microscopy over the range 100Khz to 5MHz and are represented in terms of two parameters: the initial dielectrophoretic collection rate and the initial to steady-state collection transition. Measured values for both parameters are consistent with trends in the frequency-dependent real part of the effective polarizability measured for the same plasmid DNA using dielectrophoretic spectroscopy. The experimentally measured parameters are qualitatively compared with trends predicted by theory that takes into account dielectrophoretic particle movement and diffusion. The differences between experiment and theory are discussed with suggested improvements to theoretical models, for example, including effects of electrohydronamically driven fluid motion.
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Published date: March 2006
Keywords:
AC electrokinetics, dielectrohpretic collection rate, DNA concentration, nonuniform electric fields
Organisations:
Nanoelectronics and Nanotechnology
Identifiers
Local EPrints ID: 263166
URI: http://eprints.soton.ac.uk/id/eprint/263166
PURE UUID: eb940abb-e098-47de-86c8-83132bc75d6c
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Date deposited: 08 Nov 2006
Last modified: 15 Mar 2024 03:18
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Author:
David J Bakewell
Author:
Hywel Morgan
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