The University of Southampton
University of Southampton Institutional Repository

Assembly and antigen-presenting function of MHC class I molecules in cells lacking the ER chaperone calreticulin

Record type: Article

MHC class I molecules expressed in a calreticulin-deficient cell line (K42) assembled with ? 2-microglobulin (?2-m) normally, but their subsequent loading with optimal peptides was defective. Suboptimally loaded class I molecules were released into the secretory pathway. This occurred despite the ability of newly synthesized class I to interact with the transporter associated with antigen processing (TAP) loading complex. The efficiency of peptide loading was reduced by 50%–80%, and impaired T cell recognition was observed for three out of four antigens tested. The peptide-loading function was specific to calreticulin, since the defect in K42 could be rectified by transfection with calreticulin but not a soluble form of calnexin, which shares its lectin-like activity.

Full text not available from this repository.

Citation

Gao, Bin, Adhikari, Raju, Howarth, Mark, Nakamura, Kimitoshi, Gold, Marielle C., Hill, Ann B., Knee, Rai, Michalak, Marek and Elliott, Tim (2002) Assembly and antigen-presenting function of MHC class I molecules in cells lacking the ER chaperone calreticulin Immunity, 16, (1), pp. 99-109. (doi:10.1016/S1074-7613(01)00260-6).

More information

Published date: January 2002

Identifiers

Local EPrints ID: 26319
URI: http://eprints.soton.ac.uk/id/eprint/26319
ISSN: 1097-4180
PURE UUID: 3f5e7c58-d9f7-4999-816c-882dff447163
ORCID for Tim Elliott: ORCID iD orcid.org/0000-0003-1097-0222

Catalogue record

Date deposited: 20 Apr 2006
Last modified: 17 Jul 2017 16:07

Export record

Altmetrics

Contributors

Author: Bin Gao
Author: Raju Adhikari
Author: Mark Howarth
Author: Kimitoshi Nakamura
Author: Marielle C. Gold
Author: Ann B. Hill
Author: Rai Knee
Author: Marek Michalak
Author: Tim Elliott ORCID iD

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×