Assembly and antigen-presenting function of MHC class I molecules in cells lacking the ER chaperone calreticulin


Gao, Bin, Adhikari, Raju, Howarth, Mark, Nakamura, Kimitoshi, Gold, Marielle C., Hill, Ann B., Knee, Rai, Michalak, Marek and Elliott, Tim (2002) Assembly and antigen-presenting function of MHC class I molecules in cells lacking the ER chaperone calreticulin Immunity, 16, (1), pp. 99-109. (doi:10.1016/S1074-7613(01)00260-6).

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Description/Abstract

MHC class I molecules expressed in a calreticulin-deficient cell line (K42) assembled with ? 2-microglobulin (?2-m) normally, but their subsequent loading with optimal peptides was defective. Suboptimally loaded class I molecules were released into the secretory pathway. This occurred despite the ability of newly synthesized class I to interact with the transporter associated with antigen processing (TAP) loading complex. The efficiency of peptide loading was reduced by 50%–80%, and impaired T cell recognition was observed for three out of four antigens tested. The peptide-loading function was specific to calreticulin, since the defect in K42 could be rectified by transfection with calreticulin but not a soluble form of calnexin, which shares its lectin-like activity.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1016/S1074-7613(01)00260-6
ISSNs: 1097-4180 (print)
Related URLs:
Subjects: Q Science > QR Microbiology > QR180 Immunology
ePrint ID: 26319
Date :
Date Event
January 2002Published
Date Deposited: 20 Apr 2006
Last Modified: 18 Feb 2017 16:37
Further Information:Google Scholar
URI: http://eprints.soton.ac.uk/id/eprint/26319

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