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Assembly and antigen-presenting function of MHC class I molecules in cells lacking the ER chaperone calreticulin

Assembly and antigen-presenting function of MHC class I molecules in cells lacking the ER chaperone calreticulin
Assembly and antigen-presenting function of MHC class I molecules in cells lacking the ER chaperone calreticulin
MHC class I molecules expressed in a calreticulin-deficient cell line (K42) assembled with ? 2-microglobulin (?2-m) normally, but their subsequent loading with optimal peptides was defective. Suboptimally loaded class I molecules were released into the secretory pathway. This occurred despite the ability of newly synthesized class I to interact with the transporter associated with antigen processing (TAP) loading complex. The efficiency of peptide loading was reduced by 50%–80%, and impaired T cell recognition was observed for three out of four antigens tested. The peptide-loading function was specific to calreticulin, since the defect in K42 could be rectified by transfection with calreticulin but not a soluble form of calnexin, which shares its lectin-like activity.
1097-4180
99-109
Gao, Bin
763f5d11-2809-4cea-a518-7f55037bceba
Adhikari, Raju
3510ce82-0be9-4630-bad4-6670c3f28f0e
Howarth, Mark
75054a4c-d313-4a69-ba86-8a08104ca300
Nakamura, Kimitoshi
bc7a973f-90fe-4db5-88ab-748c2f74e2ca
Gold, Marielle C.
98562b0c-18d7-4701-83a7-395832484a16
Hill, Ann B.
7c793b74-1657-4b1d-9202-2ec6c0c6216c
Knee, Rai
1662cf0d-01dd-41b8-8d71-de26734b9d2f
Michalak, Marek
b25863b7-ad2e-4c83-bd86-fd433f66be02
Elliott, Tim
16670fa8-c2f9-477a-91df-7c9e5b453e0e
Gao, Bin
763f5d11-2809-4cea-a518-7f55037bceba
Adhikari, Raju
3510ce82-0be9-4630-bad4-6670c3f28f0e
Howarth, Mark
75054a4c-d313-4a69-ba86-8a08104ca300
Nakamura, Kimitoshi
bc7a973f-90fe-4db5-88ab-748c2f74e2ca
Gold, Marielle C.
98562b0c-18d7-4701-83a7-395832484a16
Hill, Ann B.
7c793b74-1657-4b1d-9202-2ec6c0c6216c
Knee, Rai
1662cf0d-01dd-41b8-8d71-de26734b9d2f
Michalak, Marek
b25863b7-ad2e-4c83-bd86-fd433f66be02
Elliott, Tim
16670fa8-c2f9-477a-91df-7c9e5b453e0e

Gao, Bin, Adhikari, Raju, Howarth, Mark, Nakamura, Kimitoshi, Gold, Marielle C., Hill, Ann B., Knee, Rai, Michalak, Marek and Elliott, Tim (2002) Assembly and antigen-presenting function of MHC class I molecules in cells lacking the ER chaperone calreticulin Immunity, 16, (1), pp. 99-109. (doi:10.1016/S1074-7613(01)00260-6).

Record type: Article

Abstract

MHC class I molecules expressed in a calreticulin-deficient cell line (K42) assembled with ? 2-microglobulin (?2-m) normally, but their subsequent loading with optimal peptides was defective. Suboptimally loaded class I molecules were released into the secretory pathway. This occurred despite the ability of newly synthesized class I to interact with the transporter associated with antigen processing (TAP) loading complex. The efficiency of peptide loading was reduced by 50%–80%, and impaired T cell recognition was observed for three out of four antigens tested. The peptide-loading function was specific to calreticulin, since the defect in K42 could be rectified by transfection with calreticulin but not a soluble form of calnexin, which shares its lectin-like activity.

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Published date: January 2002

Identifiers

Local EPrints ID: 26319
URI: http://eprints.soton.ac.uk/id/eprint/26319
ISSN: 1097-4180
PURE UUID: 3f5e7c58-d9f7-4999-816c-882dff447163
ORCID for Tim Elliott: ORCID iD orcid.org/0000-0003-1097-0222

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Date deposited: 20 Apr 2006
Last modified: 17 Jul 2017 16:07

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Contributors

Author: Bin Gao
Author: Raju Adhikari
Author: Mark Howarth
Author: Kimitoshi Nakamura
Author: Marielle C. Gold
Author: Ann B. Hill
Author: Rai Knee
Author: Marek Michalak
Author: Tim Elliott ORCID iD

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