The University of Southampton
University of Southampton Institutional Repository

The crystal structure of H-2D(b) complexed with a partial peptide epitope suggests a major histocompatibility complex class I assembly intermediate

Glithero, Ann, Tormo, Jose, Doering, Klaus, Kojima, Mayumi, Jones, E. Yvonne and Elliott, Tim (2006) The crystal structure of H-2D(b) complexed with a partial peptide epitope suggests a major histocompatibility complex class I assembly intermediate The Journal of Biological Chemistry, 281, (18), pp. 12699-12704. (doi:10.1074/jbc.M511683200).

Record type: Article

Abstract

In the absence of bound peptide ligands, major histocompatibility complex (MHC) class I molecules are unstable. In an attempt to determine the minimum requirement for peptide-dependent MHC class I stabilization, we have used short synthetic peptides derived from the Sendai virus nucleoprotein epitope ( residues 324-332, (1)FAPGNYPAL(9)) to promote its folding in vitro of H-2D(b). We found that H-2D(b) can be stabilized by the pentapeptide (5)NYPAL(9), which is equivalent to the C-terminal portion of the optimal nonapeptide and includes both the P5 and P9 anchor residues. We have crystallized the complex of the H-2D(b) molecule with the pentamer and determined the structure to show how a quasi-stable MHC class I molecule can be formed by occupancy of a single binding pocket in the peptide-binding groove.

Full text not available from this repository.

More information

Published date: 5 May 2006
Keywords: mhc, absence, resolution, in-vitro, time, tapasin, molecules, 3-dimensional structure, expression, t-cell recognition, binding, induced conformational change, bound peptide, stability

Identifiers

Local EPrints ID: 26325
URI: http://eprints.soton.ac.uk/id/eprint/26325
ISSN: 0021-9258
PURE UUID: 8ba4f516-3743-4ada-b743-22bd8d22aa0e
ORCID for Tim Elliott: ORCID iD orcid.org/0000-0003-1097-0222

Catalogue record

Date deposited: 07 Apr 2006
Last modified: 17 Jul 2017 16:07

Export record

Altmetrics

Contributors

Author: Ann Glithero
Author: Jose Tormo
Author: Klaus Doering
Author: Mayumi Kojima
Author: E. Yvonne Jones
Author: Tim Elliott ORCID iD

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×