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Molecular cytogenetics in childhood leukemia

Molecular cytogenetics in childhood leukemia
Molecular cytogenetics in childhood leukemia
In the last decade molecular cytogenetics, or fluorescence in situ hybridization (FISH), has become an important complementary procedure to routine chromosomal analysis. The most significant consequence from cytogenetic studies in childhood leukemia has been the association of specific chromosomal abnormalities with different patient subgroups, particularly in relation to prognosis. In childhood acute myeloid leukemia (AML), the rearrangements t(8;21)(q22;q22), t(15;17)(q22;q12), and inv(16)(p13q22) are associated with a good outcome.
Conversely, deletions of the long arm of chromosome 5, monosomy 5 or 7, in association with a complex karyotype, are related to a poor prognosis. Karyotypes with a favorable outcome in childhood acute lymphoblastic leukemia (ALL) include high hyperdiploidy (51-65 chromosomes) and the translocation, t(12;21)(p13;q22). The Philadelphia translocation, t(9;22)(q34;q11), rearrangements involving the MLL gene and near haploidy (23-29 chromosomes) are associated with a short overall survival (2). Metaphase and interphase FISH are increasingly being used to screen routinely for such chromosomal abnormalities in childhood leukemia. Metaphase FISH also plays a role in the identification of new nonrandom chromosomal changes of prognostic significance.
1543-1894
123-137
Harrison, Christine J.
52da7673-509c-4b88-b92e-0c021c9c7d3e
Kempski, Helena
a2db8f50-62b6-4baf-92a8-767f40a2563a
Hammond, David W.
43d08c32-7354-4cd6-a1b4-906352d2374e
Kearney, Lyndal
ad57b9c8-b5e2-4a77-9a11-8d1e3827e298
Harrison, Christine J.
52da7673-509c-4b88-b92e-0c021c9c7d3e
Kempski, Helena
a2db8f50-62b6-4baf-92a8-767f40a2563a
Hammond, David W.
43d08c32-7354-4cd6-a1b4-906352d2374e
Kearney, Lyndal
ad57b9c8-b5e2-4a77-9a11-8d1e3827e298

Harrison, Christine J., Kempski, Helena, Hammond, David W. and Kearney, Lyndal (2004) Molecular cytogenetics in childhood leukemia. Methods in Molecular Medicine, 91, 123-137.

Record type: Article

Abstract

In the last decade molecular cytogenetics, or fluorescence in situ hybridization (FISH), has become an important complementary procedure to routine chromosomal analysis. The most significant consequence from cytogenetic studies in childhood leukemia has been the association of specific chromosomal abnormalities with different patient subgroups, particularly in relation to prognosis. In childhood acute myeloid leukemia (AML), the rearrangements t(8;21)(q22;q22), t(15;17)(q22;q12), and inv(16)(p13q22) are associated with a good outcome.
Conversely, deletions of the long arm of chromosome 5, monosomy 5 or 7, in association with a complex karyotype, are related to a poor prognosis. Karyotypes with a favorable outcome in childhood acute lymphoblastic leukemia (ALL) include high hyperdiploidy (51-65 chromosomes) and the translocation, t(12;21)(p13;q22). The Philadelphia translocation, t(9;22)(q34;q11), rearrangements involving the MLL gene and near haploidy (23-29 chromosomes) are associated with a short overall survival (2). Metaphase and interphase FISH are increasingly being used to screen routinely for such chromosomal abnormalities in childhood leukemia. Metaphase FISH also plays a role in the identification of new nonrandom chromosomal changes of prognostic significance.

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Published date: 2004

Identifiers

Local EPrints ID: 26373
URI: http://eprints.soton.ac.uk/id/eprint/26373
ISSN: 1543-1894
PURE UUID: d22ef886-5483-44f0-8153-70f0d4104093

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Date deposited: 21 Apr 2006
Last modified: 08 Jan 2022 06:54

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Contributors

Author: Christine J. Harrison
Author: Helena Kempski
Author: David W. Hammond
Author: Lyndal Kearney

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