The University of Southampton
University of Southampton Institutional Repository

Broadband single cell impedance spectroscopy using maximum length sequences: theoretical analysis and practical considerations

Sun, Tao, Gawad, Shady, Bernabini, Catia, Green, Nicolas G and Morgan, Hywel (2007) Broadband single cell impedance spectroscopy using maximum length sequences: theoretical analysis and practical considerations Measurement Science and Technology, 18, pp. 2859-2868.

Record type: Article


Measurements of the dielectric (or impedance) properties of cells can be used as a general characterization and diagnostic tool. In this paper, we describe a novel impedance spectroscopy technique for the analysis of single biological cells in suspension. The technique uses maximum length sequences (MLS) for periodic excitation signal in a microfluidic impedance cytometer. The method allows multi-frequency single cell impedance measurements to be made in a short time period (ms). Spectral information is obtained in the frequency domain by applying a fast M-sequence transform (FMT) and fast Fourier transform (FFT) to the time domain response. Theoretically, the impedance is determined from the transfer function of the system when the MLS is a current excitation. The order of the MLS and sampling rate of A/D conversion are two factors that determine the bandwidth and spectral accuracy of the technique. Experimentally, the applicability of the technique is demonstrated by characterizing the impedance spectrum of red blood cells (RBCs) in a microfluidic cytometer. The impedance is measured within 1 ms at 512 discrete frequencies, evenly distributed in the range from 976.56 Hz to 500 kHz. The measured spectrum shows good agreement with simulations.

PDF J42_Sun_Gawad_Bernabini_Green_Morgan_Measurement_Science_And_Technology_2007.pdf - Other
Download (1MB)

More information

Published date: July 2007
Organisations: Electronics & Computer Science


Local EPrints ID: 264335
ISSN: 1361-6501
PURE UUID: 64c74d37-e2d9-4fe8-9aba-2f3e1f337562
ORCID for Nicolas G Green: ORCID iD
ORCID for Hywel Morgan: ORCID iD

Catalogue record

Date deposited: 23 Jul 2007
Last modified: 21 Oct 2017 20:09

Export record


Author: Tao Sun
Author: Shady Gawad
Author: Catia Bernabini
Author: Nicolas G Green ORCID iD
Author: Hywel Morgan ORCID iD

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton:

ePrints Soton supports OAI 2.0 with a base URL of

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.