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Ultrashort electric pulse induced changes in cellular dielectric properties

Ultrashort electric pulse induced changes in cellular dielectric properties
Ultrashort electric pulse induced changes in cellular dielectric properties
The interaction of nanosecond duration pulsed electric fields (nsPEFs) with biological cells, and the models describing this behavior, depend critically on the electrical properties of the cells being pulsed. Here, we used time domain dielectric spectroscopy to measure the dielectric properties of Jurkat cells, a malignant human T-cell line, before and after exposure to five 10 ns, 150 kV/cm electrical pulses. The cytoplasm and nucleoplasm conductivities decreased dramatically following pulsing, corresponding to previously observed rises in cell suspension conductivity. This suggests that electropermeabilization occurred, resulting in ion transport from the cell’s interior to the exterior. A delayed decrease in cell membrane conductivity after the nsPEFs possibly suggests long-term ion channel damage or use dependence due to repeated membrane charging and discharging. This data could be used in models describing the phenomena at work.
Dielectric spectroscopy, Nanosecond pulsed electric fields, Electroporation, Permeabilization, Plasma membrane, Time domain, Intracellular manipulation, Ultrashort pulses
0006-291X
139-144
Garner, Allen
1ea7ee55-eff6-4f90-ae9b-b7b83f8e5e27
Chen, George
3de45a9c-6c9a-4bcb-90c3-d7e26be21819
Chen, N
8e94c68e-cc43-4b5f-b942-e8097b7843b5
Sridhara, V
ad6fb53f-985c-4159-badc-9ba2003a42d0
Kolb, J K
f2514e32-ed23-4768-9741-07a79fe602d7
Swanson, R J
2b266a1e-bae1-4f08-b762-6bee2497af6c
Beebe, S J
ecdd6d6a-c270-45c0-a0bc-2f5c25c44cf7
Joshi, R P
c99508b0-5ec1-4a76-bfb9-7e7367aebd1b
Schoenbach, K H
9ba626fa-421b-47a0-8276-6825ccd99da6
Garner, Allen
1ea7ee55-eff6-4f90-ae9b-b7b83f8e5e27
Chen, George
3de45a9c-6c9a-4bcb-90c3-d7e26be21819
Chen, N
8e94c68e-cc43-4b5f-b942-e8097b7843b5
Sridhara, V
ad6fb53f-985c-4159-badc-9ba2003a42d0
Kolb, J K
f2514e32-ed23-4768-9741-07a79fe602d7
Swanson, R J
2b266a1e-bae1-4f08-b762-6bee2497af6c
Beebe, S J
ecdd6d6a-c270-45c0-a0bc-2f5c25c44cf7
Joshi, R P
c99508b0-5ec1-4a76-bfb9-7e7367aebd1b
Schoenbach, K H
9ba626fa-421b-47a0-8276-6825ccd99da6

Garner, Allen, Chen, George, Chen, N, Sridhara, V, Kolb, J K, Swanson, R J, Beebe, S J, Joshi, R P and Schoenbach, K H (2007) Ultrashort electric pulse induced changes in cellular dielectric properties. Biochemical and Biophysical Research Communications, 362 (1), 139-144.

Record type: Article

Abstract

The interaction of nanosecond duration pulsed electric fields (nsPEFs) with biological cells, and the models describing this behavior, depend critically on the electrical properties of the cells being pulsed. Here, we used time domain dielectric spectroscopy to measure the dielectric properties of Jurkat cells, a malignant human T-cell line, before and after exposure to five 10 ns, 150 kV/cm electrical pulses. The cytoplasm and nucleoplasm conductivities decreased dramatically following pulsing, corresponding to previously observed rises in cell suspension conductivity. This suggests that electropermeabilization occurred, resulting in ion transport from the cell’s interior to the exterior. A delayed decrease in cell membrane conductivity after the nsPEFs possibly suggests long-term ion channel damage or use dependence due to repeated membrane charging and discharging. This data could be used in models describing the phenomena at work.

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Published date: October 2007
Keywords: Dielectric spectroscopy, Nanosecond pulsed electric fields, Electroporation, Permeabilization, Plasma membrane, Time domain, Intracellular manipulation, Ultrashort pulses
Organisations: Electronics & Computer Science, EEE

Identifiers

Local EPrints ID: 264453
URI: http://eprints.soton.ac.uk/id/eprint/264453
ISSN: 0006-291X
PURE UUID: 661bcdde-9842-4742-99f7-2f1e107dff62

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Date deposited: 04 Sep 2007
Last modified: 16 Dec 2019 20:42

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