Air-Exposure Technique for the Formation of Artificial Lipid Bilayers in Microsystems
Air-Exposure Technique for the Formation of Artificial Lipid Bilayers in Microsystems
To develop a reliable method for on-chip bilayer lipid membrane (BLM) formation, which could be employed for use in a biosensor array platform, a polymer microfluidic device has been constructed, and the formation of suspended BLMs within it has been investigated. A simple, yet reproducible BLM formation protocol has been developed, in which a brief air-exposure period is employed to induce the rapid thinning of an initially thick lipid-solvent layer. The technique is rapid, reproducible, and amenable to the simple injection of proteins or analytes, as well as to buffer exchange on both sides of the membrane. Scaling up the technique for use in an array platform is also straightforward, the simultaneous formation of three individually addressable BLMs being demonstrated.
8277-8284
Sandison, M.E.
fe8a3953-a4d6-4c24-a959-ea6c8ec43aed
Zagnoni, M.
c719a8d2-cd85-4d22-a669-eb0557b3aa95
Morgan, H.
de00d59f-a5a2-48c4-a99a-1d5dd7854174
2007
Sandison, M.E.
fe8a3953-a4d6-4c24-a959-ea6c8ec43aed
Zagnoni, M.
c719a8d2-cd85-4d22-a669-eb0557b3aa95
Morgan, H.
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Sandison, M.E., Zagnoni, M. and Morgan, H.
(2007)
Air-Exposure Technique for the Formation of Artificial Lipid Bilayers in Microsystems.
Langmuir, 23, .
(doi:10.1021/la7007528).
Abstract
To develop a reliable method for on-chip bilayer lipid membrane (BLM) formation, which could be employed for use in a biosensor array platform, a polymer microfluidic device has been constructed, and the formation of suspended BLMs within it has been investigated. A simple, yet reproducible BLM formation protocol has been developed, in which a brief air-exposure period is employed to induce the rapid thinning of an initially thick lipid-solvent layer. The technique is rapid, reproducible, and amenable to the simple injection of proteins or analytes, as well as to buffer exchange on both sides of the membrane. Scaling up the technique for use in an array platform is also straightforward, the simultaneous formation of three individually addressable BLMs being demonstrated.
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Published date: 2007
Organisations:
Nanoelectronics and Nanotechnology
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Local EPrints ID: 264502
URI: http://eprints.soton.ac.uk/id/eprint/264502
ISSN: 0743-7463
PURE UUID: e7abe148-eff2-4e1e-ac12-471fbb5ef5fa
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Date deposited: 14 Sep 2007
Last modified: 15 Mar 2024 03:18
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Author:
M.E. Sandison
Author:
M. Zagnoni
Author:
H. Morgan
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