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Controlled delivery of proteins into bilayer lipid membranes on chip

Record type: Article

The study and the exploitation of membrane proteins for drug screening applications requires a controllable and reliable method for their delivery into an artificial suspended membrane platform based on lab-on-a-chip technology. In this work, a polymeric device for forming lipid bilayers suitable for electrophysiology studies and biosensor applications is presented. The chip supports a single bilayer and is configured for controlled protein delivery through on-chip microfluidics. In order to demonstrate the principle of protein delivery, the potassium channel KcsA was reconstituted into proteoliposomes, which were then fused with the suspended bilayer on-chip. Fusion of single proteoliposomes with the membrane was identified electrically. Single channel conductance measurements of KcsA in the on-chip bilayer were recorded and these were compared to previously published data obtained with a conventional planar bilayer system.

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Citation

Zagnoni, Michele, Sandison, Mairi E., Marius, Phedra, Lee, Anthony G. and Morgan, Hywel (2007) Controlled delivery of proteins into bilayer lipid membranes on chip Lab on a Chip, 7, (9), pp. 1176-1183. (doi:10.1039/b703818f).

More information

Published date: September 2007
Organisations: Nanoelectronics and Nanotechnology, Electronics & Computer Science, Biological Sciences, Centre for Biological Sciences

Identifiers

Local EPrints ID: 264509
URI: http://eprints.soton.ac.uk/id/eprint/264509
ISSN: 1473-0197
PURE UUID: daee2739-918c-44a0-bd05-17eba005e413
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 15 Sep 2007
Last modified: 18 Jul 2017 07:34

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