The University of Southampton
University of Southampton Institutional Repository

Controlled delivery of proteins into bilayer lipid membranes on chip

Controlled delivery of proteins into bilayer lipid membranes on chip
Controlled delivery of proteins into bilayer lipid membranes on chip
The study and the exploitation of membrane proteins for drug screening applications requires a controllable and reliable method for their delivery into an artificial suspended membrane platform based on lab-on-a-chip technology. In this work, a polymeric device for forming lipid bilayers suitable for electrophysiology studies and biosensor applications is presented. The chip supports a single bilayer and is configured for controlled protein delivery through on-chip microfluidics. In order to demonstrate the principle of protein delivery, the potassium channel KcsA was reconstituted into proteoliposomes, which were then fused with the suspended bilayer on-chip. Fusion of single proteoliposomes with the membrane was identified electrically. Single channel conductance measurements of KcsA in the on-chip bilayer were recorded and these were compared to previously published data obtained with a conventional planar bilayer system.
1473-0197
1176-1183
Zagnoni, Michele
abfb96d5-0d3c-4b23-864b-b1212f6d5a42
Sandison, Mairi E.
90477e5a-2af6-44b1-8ea8-ae4515594c60
Marius, Phedra
f5bf8a27-2332-4d04-a45b-4b2677fcc865
Lee, Anthony G.
0891914c-e0e2-4ee1-b43e-1b70eb072d8e
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Zagnoni, Michele
abfb96d5-0d3c-4b23-864b-b1212f6d5a42
Sandison, Mairi E.
90477e5a-2af6-44b1-8ea8-ae4515594c60
Marius, Phedra
f5bf8a27-2332-4d04-a45b-4b2677fcc865
Lee, Anthony G.
0891914c-e0e2-4ee1-b43e-1b70eb072d8e
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Zagnoni, Michele, Sandison, Mairi E., Marius, Phedra, Lee, Anthony G. and Morgan, Hywel (2007) Controlled delivery of proteins into bilayer lipid membranes on chip. Lab on a Chip, 7 (9), 1176-1183. (doi:10.1039/b703818f).

Record type: Article

Abstract

The study and the exploitation of membrane proteins for drug screening applications requires a controllable and reliable method for their delivery into an artificial suspended membrane platform based on lab-on-a-chip technology. In this work, a polymeric device for forming lipid bilayers suitable for electrophysiology studies and biosensor applications is presented. The chip supports a single bilayer and is configured for controlled protein delivery through on-chip microfluidics. In order to demonstrate the principle of protein delivery, the potassium channel KcsA was reconstituted into proteoliposomes, which were then fused with the suspended bilayer on-chip. Fusion of single proteoliposomes with the membrane was identified electrically. Single channel conductance measurements of KcsA in the on-chip bilayer were recorded and these were compared to previously published data obtained with a conventional planar bilayer system.

Text
Controlled_delivery_of_proteins_into_bilayer_lipid_membranes_on_chip-2007.pdf - Other
Download (743kB)

More information

Published date: September 2007
Organisations: Nanoelectronics and Nanotechnology, Electronics & Computer Science, Biological Sciences, Centre for Biological Sciences

Identifiers

Local EPrints ID: 264509
URI: https://eprints.soton.ac.uk/id/eprint/264509
ISSN: 1473-0197
PURE UUID: daee2739-918c-44a0-bd05-17eba005e413
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 15 Sep 2007
Last modified: 07 Aug 2019 00:43

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×