Zagnoni, M., Sandison, M.E., Marius, P., Lee, A.G. and Morgan, H.
Controlled delivery of proteins into bilayer lipid membranes on chip
Lab on a chip, 7, .
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The study and the exploitation of membrane proteins for drug screening applications requires a controllable and reliable method for their delivery into an artificial suspended membrane platform based on lab-on-a-chip technology. In this work, a polymeric device for forming lipid bilayers suitable for electrophysiology studies and biosensor applications is presented. The chip supports a single bilayer and is configured for controlled protein delivery through on-chip microfluidics. In order to demonstrate the principle of protein delivery, the potassium channel KcsA was reconstituted into proteoliposomes, which were then fused with the suspended bilayer on-chip. Fusion of single proteoliposomes with the membrane was identified electrically. Single channel conductance measurements of KcsA in the on-chip bilayer were recorded and these were compared to previously published data obtained with a conventional planar bilayer system.
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Controlled delivery of proteins into bilayer lipid membranes on chip (deposited 15 Sep 2007)
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