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Rapid rotation of micron and submicron dielectric particles measured using optical tweezers

Rapid rotation of micron and submicron dielectric particles measured using optical tweezers
Rapid rotation of micron and submicron dielectric particles measured using optical tweezers
We demonstrate the use of a laser trap (‘optical tweezers’) and back-focal-plane position detector to measure rapid rotation in aqueous solution of single particles with sizes in the vicinity of 1 μm. Two types of rotation were measured: electrorotation of polystyrene microspheres and rotation of the flagellar motor of the bacterium Vibrio alginolyticus. In both cases, speeds in excess of 1000 Hz (rev s−1) were measured. Polystyrene beads of diameter about 1 μm labelled with smaller beads were held at the centre of a microelectrode array by the optical tweezers. Electrorotation of the labelled beads was induced by applying a rotating electric field to the solution using microelectrodes. Electrorotation spectra were obtained by varying the frequency of the applied field and analysed to obtain the surface conductance of the beads. Single cells of V. alginolyticus were trapped and rotation of the polar sodium-driven flagellar motor was measured. Cells rotated more rapidly in media containing higher concentrations of Na+, and photodamage caused by the trap was considerably less when the suspending medium did not contain oxygen. The technique allows single-speed measurements to be made in less than a second and separate particles can be measured at a rate of several per minute.
0950-0340
1539-1554
Rowe, Alexander D.
43544f0e-8975-4e86-a763-2558ac4a27c4
Leake, Mark C.
bb053fa6-bc38-4abf-ba49-fc787b6cef7c
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Berry, Richard M.
00de5be0-5905-4dc3-9a9a-c274108e6ccf
Rowe, Alexander D.
43544f0e-8975-4e86-a763-2558ac4a27c4
Leake, Mark C.
bb053fa6-bc38-4abf-ba49-fc787b6cef7c
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Berry, Richard M.
00de5be0-5905-4dc3-9a9a-c274108e6ccf

Rowe, Alexander D., Leake, Mark C., Morgan, Hywel and Berry, Richard M. (2003) Rapid rotation of micron and submicron dielectric particles measured using optical tweezers. Journal of Modern Optics, 50 (10), 1539-1554. (doi:10.1080/09500340308235228).

Record type: Article

Abstract

We demonstrate the use of a laser trap (‘optical tweezers’) and back-focal-plane position detector to measure rapid rotation in aqueous solution of single particles with sizes in the vicinity of 1 μm. Two types of rotation were measured: electrorotation of polystyrene microspheres and rotation of the flagellar motor of the bacterium Vibrio alginolyticus. In both cases, speeds in excess of 1000 Hz (rev s−1) were measured. Polystyrene beads of diameter about 1 μm labelled with smaller beads were held at the centre of a microelectrode array by the optical tweezers. Electrorotation of the labelled beads was induced by applying a rotating electric field to the solution using microelectrodes. Electrorotation spectra were obtained by varying the frequency of the applied field and analysed to obtain the surface conductance of the beads. Single cells of V. alginolyticus were trapped and rotation of the polar sodium-driven flagellar motor was measured. Cells rotated more rapidly in media containing higher concentrations of Na+, and photodamage caused by the trap was considerably less when the suspending medium did not contain oxygen. The technique allows single-speed measurements to be made in less than a second and separate particles can be measured at a rate of several per minute.

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Published date: 2003
Organisations: Nanoelectronics and Nanotechnology

Identifiers

Local EPrints ID: 264526
URI: https://eprints.soton.ac.uk/id/eprint/264526
ISSN: 0950-0340
PURE UUID: 11821a46-62bc-4ff9-b701-17351af21a83
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 19 Sep 2007
Last modified: 20 Aug 2019 00:36

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Contributors

Author: Alexander D. Rowe
Author: Mark C. Leake
Author: Hywel Morgan ORCID iD
Author: Richard M. Berry

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