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High throughput particle analysis: combining dielectrophoretic particle focussing with confocal optical detection

High throughput particle analysis: combining dielectrophoretic particle focussing with confocal optical detection
High throughput particle analysis: combining dielectrophoretic particle focussing with confocal optical detection
A microflow cytometer has been fabricated that detects and counts fluorescent particles flowing through a microchannel at a high speed based upon their fluorescence emission intensity. Dielectrophoresis is used to continuously focus particles within the flowing fluid stream into the centre of the device, which is 40 μm high and 250 μm wide. The method ensures that all the particules pass through an interrogation region approximately 5 μm in diameter, which is created by focusing a beam of light into a spot. The functioning of the device was demonstrated by detecting and counting fluorescent latex particles at a rate of up to 250 particles/s. A mixture of three different populations of latex particle was used, each sub-population with a distinct level of fluorescent intensity. The device was evaluated by comparison with a conventional fluorescent activated cell sorter (FACS) and numerical simulation demonstrated that for 6 mico m beads, and for this design of chip the theoretical throughput is of the order of 1000 particles/s (corresponding to a particle velocty of 1 mm/s).
AC electrokinetics, Dielectrophoresis, Particle focussing, Confocal detection, FACS
0956-5663
1621-1630
Holmes, David
eeff86f7-ab4b-4795-9a01-82ce1275e34f
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Green, Nicolas G.
d9b47269-c426-41fd-a41d-5f4579faa581
Holmes, David
eeff86f7-ab4b-4795-9a01-82ce1275e34f
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Green, Nicolas G.
d9b47269-c426-41fd-a41d-5f4579faa581

Holmes, David, Morgan, Hywel and Green, Nicolas G. (2006) High throughput particle analysis: combining dielectrophoretic particle focussing with confocal optical detection. Biosensors & Bioelectronics, 21, 1621-1630. (doi:10.1016/j.bios.2005.10.017).

Record type: Article

Abstract

A microflow cytometer has been fabricated that detects and counts fluorescent particles flowing through a microchannel at a high speed based upon their fluorescence emission intensity. Dielectrophoresis is used to continuously focus particles within the flowing fluid stream into the centre of the device, which is 40 μm high and 250 μm wide. The method ensures that all the particules pass through an interrogation region approximately 5 μm in diameter, which is created by focusing a beam of light into a spot. The functioning of the device was demonstrated by detecting and counting fluorescent latex particles at a rate of up to 250 particles/s. A mixture of three different populations of latex particle was used, each sub-population with a distinct level of fluorescent intensity. The device was evaluated by comparison with a conventional fluorescent activated cell sorter (FACS) and numerical simulation demonstrated that for 6 mico m beads, and for this design of chip the theoretical throughput is of the order of 1000 particles/s (corresponding to a particle velocty of 1 mm/s).

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More information

Published date: 2006
Keywords: AC electrokinetics, Dielectrophoresis, Particle focussing, Confocal detection, FACS
Organisations: Electronics & Computer Science

Identifiers

Local EPrints ID: 264531
URI: https://eprints.soton.ac.uk/id/eprint/264531
ISSN: 0956-5663
PURE UUID: f7819741-435e-4e5b-9045-95068969cb5a
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676
ORCID for Nicolas G. Green: ORCID iD orcid.org/0000-0001-9230-4455

Catalogue record

Date deposited: 19 Sep 2007
Last modified: 15 Aug 2019 00:45

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