IL-15 drives the specific migration of CD94+ and TCR-??+ intraepithelial lymphocytes in organ cultures of treated celiac patients
IL-15 drives the specific migration of CD94+ and TCR-??+ intraepithelial lymphocytes in organ cultures of treated celiac patients
Objectives: Celiac disease (CD) is an under-diagnosed but extremely frequent disease, triggered by the ingestion of gliadin. The pathogenic mechanisms of CD are still poorly understood, but intraepithelial lymphocytes are considered to have a key role. We intended to define the subsets of T lymphocytes migrating upon gliadin challenge in organ cultures of treated celiac patients and establish the type of factor(s) driving such an infiltration.
Methods: Duodenum biopsies from 10 treated celiacs and 7 controls were cultured in vitro with/without gliadin digest (1 mg/ml) or interleukin (IL)-15 (10 ng/ml). In 7 treated celiacs IL-7, IL-4, and IL-2 were similarly tested. Intraepithelial CD3, CD8, TCR-??, and CD94 were detected by immunohistochemistry and numbered per mm epithelium.
Results: IL-15 but not IL-7, IL-4, or IL-2 induced intraepithelial increase of CD3+ and CD8+ cells in celiac and control intestine (p< 0.001 vs cultures with medium). IL-15 induced increases in the number of intraepithelial TCR-??+ and CD94+ cells only in celiacs (p< 0.001). IL-7 was also effective in increasing intraepithelial TCR-??+ (but not CD94+) cells in celiac biopsies (p< 0.001). Gliadin induced intraepithelial migration of CD3+, CD8+ (p< 0.001), and CD94+ (p< 0.05) cells in celiacs, but not in controls.
Conclusions: The results we describe in this report indicate that IL-15 might have a key role in modulating and driving intraepithelial infiltration and ultimately in the pathogenesis of CD.
150-156
Maiuri, Luigi
999bc98b-70b2-4b19-ad2e-788f7f531f61
Ciacci, Carolina
36f48a3e-0ee5-49a0-8eb8-fb70af92b480
Vacca, Loredana
18e0647b-0cc0-400b-abaf-bbbbcae27211
Ricciardelli, Ida
96d57da0-8912-42aa-8610-c31a15e981a1
Auricchio, Salvatore
b1497e90-3a9e-4b91-aced-f45d51531107
Quaratino, Sonia
a17d78fe-6c03-4775-83e3-53f9d511ae70
Londei, Marco
8e3daa14-6b85-45b8-bac9-c93b05483434
2001
Maiuri, Luigi
999bc98b-70b2-4b19-ad2e-788f7f531f61
Ciacci, Carolina
36f48a3e-0ee5-49a0-8eb8-fb70af92b480
Vacca, Loredana
18e0647b-0cc0-400b-abaf-bbbbcae27211
Ricciardelli, Ida
96d57da0-8912-42aa-8610-c31a15e981a1
Auricchio, Salvatore
b1497e90-3a9e-4b91-aced-f45d51531107
Quaratino, Sonia
a17d78fe-6c03-4775-83e3-53f9d511ae70
Londei, Marco
8e3daa14-6b85-45b8-bac9-c93b05483434
Maiuri, Luigi, Ciacci, Carolina, Vacca, Loredana, Ricciardelli, Ida, Auricchio, Salvatore, Quaratino, Sonia and Londei, Marco
(2001)
IL-15 drives the specific migration of CD94+ and TCR-??+ intraepithelial lymphocytes in organ cultures of treated celiac patients.
American Journal of Gastroenterology, 96 (1), .
(doi:10.1111/j.1572-0241.2001.03437.x).
Abstract
Objectives: Celiac disease (CD) is an under-diagnosed but extremely frequent disease, triggered by the ingestion of gliadin. The pathogenic mechanisms of CD are still poorly understood, but intraepithelial lymphocytes are considered to have a key role. We intended to define the subsets of T lymphocytes migrating upon gliadin challenge in organ cultures of treated celiac patients and establish the type of factor(s) driving such an infiltration.
Methods: Duodenum biopsies from 10 treated celiacs and 7 controls were cultured in vitro with/without gliadin digest (1 mg/ml) or interleukin (IL)-15 (10 ng/ml). In 7 treated celiacs IL-7, IL-4, and IL-2 were similarly tested. Intraepithelial CD3, CD8, TCR-??, and CD94 were detected by immunohistochemistry and numbered per mm epithelium.
Results: IL-15 but not IL-7, IL-4, or IL-2 induced intraepithelial increase of CD3+ and CD8+ cells in celiac and control intestine (p< 0.001 vs cultures with medium). IL-15 induced increases in the number of intraepithelial TCR-??+ and CD94+ cells only in celiacs (p< 0.001). IL-7 was also effective in increasing intraepithelial TCR-??+ (but not CD94+) cells in celiac biopsies (p< 0.001). Gliadin induced intraepithelial migration of CD3+, CD8+ (p< 0.001), and CD94+ (p< 0.05) cells in celiacs, but not in controls.
Conclusions: The results we describe in this report indicate that IL-15 might have a key role in modulating and driving intraepithelial infiltration and ultimately in the pathogenesis of CD.
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Published date: 2001
Identifiers
Local EPrints ID: 26455
URI: http://eprints.soton.ac.uk/id/eprint/26455
ISSN: 0002-9270
PURE UUID: 53127482-702c-497f-a330-3bfbff811f76
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Date deposited: 21 Apr 2006
Last modified: 15 Mar 2024 07:10
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Contributors
Author:
Luigi Maiuri
Author:
Carolina Ciacci
Author:
Loredana Vacca
Author:
Ida Ricciardelli
Author:
Salvatore Auricchio
Author:
Sonia Quaratino
Author:
Marco Londei
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