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High speed multi-frequency impedance analysis of single biological particle using Maximum Length Sequences

High speed multi-frequency impedance analysis of single biological particle using Maximum Length Sequences
High speed multi-frequency impedance analysis of single biological particle using Maximum Length Sequences
A novel impedance spectroscopy technique has been developed for high speed single biological particle analysis. A microfluidic cytometer is used to measure the impedance of single micrometre sized latex particles at high speed across a range of frequencies. The setup uses a technique based on maximum length sequence (MLS) analysis, where the time-dependent response of the system is measured in the time domain and transformed into the impulse response using fast M-sequence transform (FMT). Finally fast Fourier transform (FFT) is applied to the impulse response to give the transfer-function of the system in the frequency domain. It is demonstrated that the MLS technique can give multi-frequency (broad-band) measurement in a short time period (ms). The impedance spectra of polystyrene micro-beads are measured at 512 evenly distributed frequencies over a range from 976.5625 Hz to 500 kHz. The spectral information for each bead is obtained in approximately 1 ms. Good agreement is shown between the MLS data and both circuit simulations and conventional AC single frequency measurements.
1086-1088
Sun, Tao
b2f8e932-a7e6-4fe7-94dd-5c4ce725eacb
Holmes, David
eeff86f7-ab4b-4795-9a01-82ce1275e34f
Gawad, Shady
98f746a7-4bcc-42bb-a450-dafda85be29c
Green, Nicolas G.
d9b47269-c426-41fd-a41d-5f4579faa581
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Sun, Tao
b2f8e932-a7e6-4fe7-94dd-5c4ce725eacb
Holmes, David
eeff86f7-ab4b-4795-9a01-82ce1275e34f
Gawad, Shady
98f746a7-4bcc-42bb-a450-dafda85be29c
Green, Nicolas G.
d9b47269-c426-41fd-a41d-5f4579faa581
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Sun, Tao, Holmes, David, Gawad, Shady, Green, Nicolas G. and Morgan, Hywel (2007) High speed multi-frequency impedance analysis of single biological particle using Maximum Length Sequences. 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2007), France. 07 - 11 Oct 2007. pp. 1086-1088 .

Record type: Conference or Workshop Item (Paper)

Abstract

A novel impedance spectroscopy technique has been developed for high speed single biological particle analysis. A microfluidic cytometer is used to measure the impedance of single micrometre sized latex particles at high speed across a range of frequencies. The setup uses a technique based on maximum length sequence (MLS) analysis, where the time-dependent response of the system is measured in the time domain and transformed into the impulse response using fast M-sequence transform (FMT). Finally fast Fourier transform (FFT) is applied to the impulse response to give the transfer-function of the system in the frequency domain. It is demonstrated that the MLS technique can give multi-frequency (broad-band) measurement in a short time period (ms). The impedance spectra of polystyrene micro-beads are measured at 512 evenly distributed frequencies over a range from 976.5625 Hz to 500 kHz. The spectral information for each bead is obtained in approximately 1 ms. Good agreement is shown between the MLS data and both circuit simulations and conventional AC single frequency measurements.

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More information

Published date: 2007
Venue - Dates: 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2007), France, 2007-10-07 - 2007-10-11
Organisations: Nanoelectronics and Nanotechnology

Identifiers

Local EPrints ID: 264674
URI: https://eprints.soton.ac.uk/id/eprint/264674
PURE UUID: 0dea477b-fcf0-4caa-a36a-368627aa5488
ORCID for Nicolas G. Green: ORCID iD orcid.org/0000-0001-9230-4455
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 14 Oct 2007
Last modified: 06 Jun 2018 12:45

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Contributors

Author: Tao Sun
Author: David Holmes
Author: Shady Gawad
Author: Hywel Morgan ORCID iD

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