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Biophysical studies of a transmembrane peptide derived from the T cell antigen receptor

Biophysical studies of a transmembrane peptide derived from the T cell antigen receptor
Biophysical studies of a transmembrane peptide derived from the T cell antigen receptor
Core peptide (CP) is a unique peptide derived from the transmembrane sequence of T cell antigen receptor (TCR)-alpha chain and is capable of inhibiting the immune response both invitro and in animal models of T cell mediated inflammation. The structure of CP, with sequence GLRILLLKV, is similar to the amphipathic region of many peptides. Unlike antimicrobial peptides, however, which damage cell membranes, electron microscopy and propidium iodide exclusion assays on cell membranes suggest that CP does not create pores and may act by interfering with signal transduction at the membrane level. To investigate this effect further we report the results of31P and2H solid-state NMR spectroscopy of CP on model membranes. As predicted, even at high concentrations of CP, the structure of model membranes was not significantly perturbed. Only at the very high peptide-to-lipid molar ratio of 1?10 significant effects on the model membranes were observed. We conclude that CP does not destroy the integrity of the lipid bilayer.
2H NMR - 31P NMR - T cell antigen receptor - CD - lipid bilayer
1573-3149
227-233
Ali, M
9bf93f22-dd46-43d1-a824-9af2e4c8eba8
de Planque, M R R
a1d33d13-f516-44fb-8d2c-c51d18bc21ba
Huynh, N T
a4166c7e-59ac-4aa4-9463-5b5dff2cb1ac
Manolios, N
48642c30-aabe-49f5-9643-301b3bac2a68
Separovic, F
1ca03f7a-0a45-465f-a683-33928d272fcd
Ali, M
9bf93f22-dd46-43d1-a824-9af2e4c8eba8
de Planque, M R R
a1d33d13-f516-44fb-8d2c-c51d18bc21ba
Huynh, N T
a4166c7e-59ac-4aa4-9463-5b5dff2cb1ac
Manolios, N
48642c30-aabe-49f5-9643-301b3bac2a68
Separovic, F
1ca03f7a-0a45-465f-a683-33928d272fcd

Ali, M, de Planque, M R R, Huynh, N T, Manolios, N and Separovic, F (2002) Biophysical studies of a transmembrane peptide derived from the T cell antigen receptor. International Journal of Peptide Research and Therapeutics, 8 (3-5), 227-233. (doi:10.1007/BF02446521).

Record type: Article

Abstract

Core peptide (CP) is a unique peptide derived from the transmembrane sequence of T cell antigen receptor (TCR)-alpha chain and is capable of inhibiting the immune response both invitro and in animal models of T cell mediated inflammation. The structure of CP, with sequence GLRILLLKV, is similar to the amphipathic region of many peptides. Unlike antimicrobial peptides, however, which damage cell membranes, electron microscopy and propidium iodide exclusion assays on cell membranes suggest that CP does not create pores and may act by interfering with signal transduction at the membrane level. To investigate this effect further we report the results of31P and2H solid-state NMR spectroscopy of CP on model membranes. As predicted, even at high concentrations of CP, the structure of model membranes was not significantly perturbed. Only at the very high peptide-to-lipid molar ratio of 1?10 significant effects on the model membranes were observed. We conclude that CP does not destroy the integrity of the lipid bilayer.

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More information

Published date: 2002
Keywords: 2H NMR - 31P NMR - T cell antigen receptor - CD - lipid bilayer
Organisations: Nanoelectronics and Nanotechnology

Identifiers

Local EPrints ID: 264695
URI: http://eprints.soton.ac.uk/id/eprint/264695
ISSN: 1573-3149
PURE UUID: 85215020-a4c5-4ca9-96bb-b460afa44403
ORCID for M R R de Planque: ORCID iD orcid.org/0000-0002-8787-0513

Catalogue record

Date deposited: 17 Oct 2007
Last modified: 18 Feb 2021 17:10

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