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Label-Free Differential Leukocyte Counts Using a Microfabricated, Single-Cell Impedance Spectrometer

Record type: Conference or Workshop Item (Paper)

Impedance spectroscopy is a non-invasive technique which allows analysis of the electrical properties of biological cells and other materials. Typically, the electrical properties of a cell suspension are measured using relatively large volumes (>100 ?l). This approach suffers from the significant drawback that the measurement is averaged over several million cells, and as such, discrimination of cell sub-population and cell sorting is impossible. We have developed a microfluidic device capable of high-speed impedance analysis of individual cells in rapid succession. In this paper, we outline the theory for impedance analysis of a single cell in a microchip. We show experimentally, the ability of the technology to distinguish, based on impedance alone, the three main leukocyte (white blood cell) sub-populations (monocytes, lymphocytes, neutrophils) in human blood. Fluorescence analysis of the cells is performed simultaneously with impedance measurement, as cells flow through the device. This provides a direct correlation between cell type (based on antibody) and impedance properties. The data shows distinct differences in the impedance properties of leukocyte sub-populations at specific frequencies.

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Citation

Holmes, David, Sun, Tao, Holloway, Judith, Cakebread, Julie, Davies, Donna and Morgan, Hywel (2007) Label-Free Differential Leukocyte Counts Using a Microfabricated, Single-Cell Impedance Spectrometer At The 6th Annual IEEE Conference on Sensors, United States. 28 - 31 Oct 2007. , pp. 1452-1456.

More information

Published date: 2007
Additional Information: Event Dates: October 28 - 31, 2007
Venue - Dates: The 6th Annual IEEE Conference on Sensors, United States, 2007-10-28 - 2007-10-31
Organisations: Nanoelectronics and Nanotechnology

Identifiers

Local EPrints ID: 264784
URI: http://eprints.soton.ac.uk/id/eprint/264784
PURE UUID: 47390829-1551-4210-aa31-7dff017bbb68
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 05 Nov 2007
Last modified: 18 Jul 2017 07:32

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Contributors

Author: David Holmes
Author: Tao Sun
Author: Judith Holloway
Author: Julie Cakebread
Author: Donna Davies
Author: Hywel Morgan ORCID iD

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