Heterogeneity of VH-JH gene rearrangement patterns: an insight into the biology of B cell precursor ALL
Heterogeneity of VH-JH gene rearrangement patterns: an insight into the biology of B cell precursor ALL
Oligoclonal B cell proliferation, as defined by the presence of more than one leukemic clone, has been detected in approximately 20% to 30% of patients with acute lymphoblastic leukemia (ALL) using PCR or Southern blotting. An accurate assessment of these populations is required to avoid false negative measurements of minimal residual disease (MRD) in follow-up bone marrow (BM) samples of ALL patients. In this study, we analysed 29 ALL patients with two or more immunoglobulin heavy (IGH) chain gene rearrangements in the presentation samples using IGH fingerprinting PCR and sequence analysis. Thirty-nine (51%) of 76 sequences (from 15 patients), shared no VNDNJ homology (ie different CDR3 regions). In the remaining 14 patients, at least two related VH sequences were identified in each patient (identical DNJ sequences). Numerical abnormalities of chromosome 14 was detected in 10 patients. Eight patients were analysed at presentation and relapse. In four of them, expansion of a minor presentation-clone was detected at relapse while the major presentation clone disappeared, confirming 'subclonal evolution'. Finally, in our cohort of patients, the presence of related or unrelated IGHclones did not influence overall survival.
1527-1536
Moreira, I.
266f0ae4-8050-4699-a440-6a76487b1a34
Papaioannou, M.
c3777e36-6587-4489-93b0-e747aa6c378f
Mortuza, F.Y.
3b12b235-810a-4bb8-adcc-687ac0b8177c
Gameiro, P.
d8d788f8-47f9-4175-bbdc-6cb383126bd4
Palmisano, G.L.
60bcbca1-e5dc-49e6-b863-fbf84a366de9
Harrison, C.J.
9c9f6b47-8bfc-49dd-b156-74539b170291
Prentice, H.G.
9f2b983d-671a-44bb-9820-08c3b7f64d69
Mehta, A.B.A.V.
6a058f49-e342-4d71-87b0-6b2b240d3d47
Foroni, L.
dd5521ed-04aa-4049-9624-7eaef86dec43
2001
Moreira, I.
266f0ae4-8050-4699-a440-6a76487b1a34
Papaioannou, M.
c3777e36-6587-4489-93b0-e747aa6c378f
Mortuza, F.Y.
3b12b235-810a-4bb8-adcc-687ac0b8177c
Gameiro, P.
d8d788f8-47f9-4175-bbdc-6cb383126bd4
Palmisano, G.L.
60bcbca1-e5dc-49e6-b863-fbf84a366de9
Harrison, C.J.
9c9f6b47-8bfc-49dd-b156-74539b170291
Prentice, H.G.
9f2b983d-671a-44bb-9820-08c3b7f64d69
Mehta, A.B.A.V.
6a058f49-e342-4d71-87b0-6b2b240d3d47
Foroni, L.
dd5521ed-04aa-4049-9624-7eaef86dec43
Moreira, I., Papaioannou, M., Mortuza, F.Y., Gameiro, P., Palmisano, G.L., Harrison, C.J., Prentice, H.G., Mehta, A.B.A.V. and Foroni, L.
(2001)
Heterogeneity of VH-JH gene rearrangement patterns: an insight into the biology of B cell precursor ALL.
Leukemia, 15 (10), .
Abstract
Oligoclonal B cell proliferation, as defined by the presence of more than one leukemic clone, has been detected in approximately 20% to 30% of patients with acute lymphoblastic leukemia (ALL) using PCR or Southern blotting. An accurate assessment of these populations is required to avoid false negative measurements of minimal residual disease (MRD) in follow-up bone marrow (BM) samples of ALL patients. In this study, we analysed 29 ALL patients with two or more immunoglobulin heavy (IGH) chain gene rearrangements in the presentation samples using IGH fingerprinting PCR and sequence analysis. Thirty-nine (51%) of 76 sequences (from 15 patients), shared no VNDNJ homology (ie different CDR3 regions). In the remaining 14 patients, at least two related VH sequences were identified in each patient (identical DNJ sequences). Numerical abnormalities of chromosome 14 was detected in 10 patients. Eight patients were analysed at presentation and relapse. In four of them, expansion of a minor presentation-clone was detected at relapse while the major presentation clone disappeared, confirming 'subclonal evolution'. Finally, in our cohort of patients, the presence of related or unrelated IGHclones did not influence overall survival.
This record has no associated files available for download.
More information
Published date: 2001
Identifiers
Local EPrints ID: 26498
URI: http://eprints.soton.ac.uk/id/eprint/26498
ISSN: 0887-6924
PURE UUID: 442377a0-bdcf-46a2-83ff-a3c3d0b16b9f
Catalogue record
Date deposited: 12 Apr 2006
Last modified: 08 Jan 2022 03:50
Export record
Contributors
Author:
I. Moreira
Author:
M. Papaioannou
Author:
F.Y. Mortuza
Author:
P. Gameiro
Author:
G.L. Palmisano
Author:
C.J. Harrison
Author:
H.G. Prentice
Author:
A.B.A.V. Mehta
Author:
L. Foroni
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics