The University of Southampton
University of Southampton Institutional Repository

Microfluidic system for cell transfection using sonoporation and ultrasonic particle manipulation

Microfluidic system for cell transfection using sonoporation and ultrasonic particle manipulation
Microfluidic system for cell transfection using sonoporation and ultrasonic particle manipulation
Studies into sonoporation have grown rapidly in biotechnology and medicine in recent years. The use of sonoporation in biotechnology and medicine has potential for enhancing and targeting administration of drugs genes and other therapeutic compounds into the cells. Sonoporation has demonstrated to facilitate efficient gene transfer both in vitro and in vivo. This paper presents a microfluidic system for cells transfection using sonoporation and ultrasonic particle manipulation. The paper presents a theoretical analysis used to design the sonoporation chamber, whilst also enabling ultrasonic particle manipulation using an ultrasonic standing wave. Both the sonoporation and the particle manipulation are achieved using the same piezoelectric transducer (PZT 26) mounted on the micro chamber. The theoretical analysis is based on upon the model for an acoustic particle separator. The resulting design has been implemented in Macor ceramic glass assembly with a chamber size of 6 mm diameter and 750?m thickness. The efficiency of the sonoporation system was determined experimentally by the use of HeLa cells and propidium dye. The transfection rate was determined under a range of sonoporation conditions. The optimal sonoporation parameters will vary depending upon the cell type and purpose. For example, sonoporation typically utilises ultrasonic frequencies of 1 to 3MHz for gene delivery and drug delivery. The experiment results showed a peak cell transfection efficiency of 52.2% at 1.17MHz at 80 Vp-p.
57
Rodamporn, Somphop
e06728a0-30ec-43bc-a95c-677265248eef
Beeby, Steve
ba565001-2812-4300-89f1-fe5a437ecb0d
Harris, Nick
237cfdbd-86e4-4025-869c-c85136f14dfd
Brown, Andrew
5c19e523-65ec-499b-9e7c-91522017d7e0
Hill, Martin
db8efb37-0c44-4321-b4cb-f253dd3f50f1
Chad, John
2b52a3e0-a9e1-4988-996b-132e95ba38bd
Rodamporn, Somphop
e06728a0-30ec-43bc-a95c-677265248eef
Beeby, Steve
ba565001-2812-4300-89f1-fe5a437ecb0d
Harris, Nick
237cfdbd-86e4-4025-869c-c85136f14dfd
Brown, Andrew
5c19e523-65ec-499b-9e7c-91522017d7e0
Hill, Martin
db8efb37-0c44-4321-b4cb-f253dd3f50f1
Chad, John
2b52a3e0-a9e1-4988-996b-132e95ba38bd

Rodamporn, Somphop, Beeby, Steve, Harris, Nick, Brown, Andrew, Hill, Martin and Chad, John (2007) Microfluidic system for cell transfection using sonoporation and ultrasonic particle manipulation At International Conference on Cellular & Molecular Bioengineering. 10 - 12 Dec 2007. , p. 57.

Record type: Conference or Workshop Item (Other)

Abstract

Studies into sonoporation have grown rapidly in biotechnology and medicine in recent years. The use of sonoporation in biotechnology and medicine has potential for enhancing and targeting administration of drugs genes and other therapeutic compounds into the cells. Sonoporation has demonstrated to facilitate efficient gene transfer both in vitro and in vivo. This paper presents a microfluidic system for cells transfection using sonoporation and ultrasonic particle manipulation. The paper presents a theoretical analysis used to design the sonoporation chamber, whilst also enabling ultrasonic particle manipulation using an ultrasonic standing wave. Both the sonoporation and the particle manipulation are achieved using the same piezoelectric transducer (PZT 26) mounted on the micro chamber. The theoretical analysis is based on upon the model for an acoustic particle separator. The resulting design has been implemented in Macor ceramic glass assembly with a chamber size of 6 mm diameter and 750?m thickness. The efficiency of the sonoporation system was determined experimentally by the use of HeLa cells and propidium dye. The transfection rate was determined under a range of sonoporation conditions. The optimal sonoporation parameters will vary depending upon the cell type and purpose. For example, sonoporation typically utilises ultrasonic frequencies of 1 to 3MHz for gene delivery and drug delivery. The experiment results showed a peak cell transfection efficiency of 52.2% at 1.17MHz at 80 Vp-p.

PDF abstract_ICCMB2007.pdf - Other
Download (56kB)

More information

Published date: 10 December 2007
Additional Information: Event Dates: 10-12 December 2007
Venue - Dates: International Conference on Cellular & Molecular Bioengineering, 2007-12-10 - 2007-12-12
Organisations: EEE

Identifiers

Local EPrints ID: 265023
URI: http://eprints.soton.ac.uk/id/eprint/265023
PURE UUID: a813c8a6-9564-4252-9bcc-ce90f51ee1dc
ORCID for Steve Beeby: ORCID iD orcid.org/0000-0002-0800-1759
ORCID for Nick Harris: ORCID iD orcid.org/0000-0003-4122-2219

Catalogue record

Date deposited: 10 Jan 2008 14:27
Last modified: 18 Jul 2017 07:30

Export record

Contributors

Author: Somphop Rodamporn
Author: Steve Beeby ORCID iD
Author: Nick Harris ORCID iD
Author: Andrew Brown
Author: Martin Hill
Author: John Chad

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×