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Doping of carbon nanotubes with nitrogen improves protein coverage whilst retaining correct conformation

Doping of carbon nanotubes with nitrogen improves protein coverage whilst retaining correct conformation
Doping of carbon nanotubes with nitrogen improves protein coverage whilst retaining correct conformation
Relevant parameters for non-covalent protein functionalization of carbon nanotubes are explored. Multiwalled carbon nanotubes are carboxylated and functionalized with metalloproteins. Using atomic force microscopy (AFM) we quantitatively determine that coverage with nitrogen-doped multiwalled carbon nanotubes is superior compared to coverage with un-doped multiwalled carbon nanotubes, due to enhanced carboxylation. Conformational analysis using a combination of AFM, antibody binding assays, circular dichroism and UV–visible spectroscopy demonstrates that the metalloproteins retain their native structure when adsorbed to nitrogen-doped multiwalled carbon nanotubes irrespective of their size, charge or folding motif.
0957-4484
384001
Burch, Hilary J.
5bd30101-4b6b-4ebd-9566-5acbbec8789d
Antoranz Contera, Sonia
cda040e3-6ec7-4761-8e93-525ab65c8237
de Planque, Maurits R.R.
a1d33d13-f516-44fb-8d2c-c51d18bc21ba
Grobert, Nicole
b73e5061-1588-4814-84a3-d849b2162122
Ryan, John F.
53fffc6b-840d-445b-a852-9418834071ab
Burch, Hilary J.
5bd30101-4b6b-4ebd-9566-5acbbec8789d
Antoranz Contera, Sonia
cda040e3-6ec7-4761-8e93-525ab65c8237
de Planque, Maurits R.R.
a1d33d13-f516-44fb-8d2c-c51d18bc21ba
Grobert, Nicole
b73e5061-1588-4814-84a3-d849b2162122
Ryan, John F.
53fffc6b-840d-445b-a852-9418834071ab

Burch, Hilary J., Antoranz Contera, Sonia, de Planque, Maurits R.R., Grobert, Nicole and Ryan, John F. (2008) Doping of carbon nanotubes with nitrogen improves protein coverage whilst retaining correct conformation. Nanotechnology, 19 (38), 384001. (doi:10.1088/0957-4484/19/38/384001).

Record type: Article

Abstract

Relevant parameters for non-covalent protein functionalization of carbon nanotubes are explored. Multiwalled carbon nanotubes are carboxylated and functionalized with metalloproteins. Using atomic force microscopy (AFM) we quantitatively determine that coverage with nitrogen-doped multiwalled carbon nanotubes is superior compared to coverage with un-doped multiwalled carbon nanotubes, due to enhanced carboxylation. Conformational analysis using a combination of AFM, antibody binding assays, circular dichroism and UV–visible spectroscopy demonstrates that the metalloproteins retain their native structure when adsorbed to nitrogen-doped multiwalled carbon nanotubes irrespective of their size, charge or folding motif.

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More information

Published date: 12 August 2008
Organisations: Nanoelectronics and Nanotechnology

Identifiers

Local EPrints ID: 265230
URI: https://eprints.soton.ac.uk/id/eprint/265230
ISSN: 0957-4484
PURE UUID: 16b96e0f-c742-4023-8434-b1da52a6d8d4
ORCID for Maurits R.R. de Planque: ORCID iD orcid.org/0000-0002-8787-0513

Catalogue record

Date deposited: 29 Feb 2008 15:42
Last modified: 26 Nov 2019 01:43

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Contributors

Author: Hilary J. Burch
Author: Sonia Antoranz Contera
Author: Maurits R.R. de Planque ORCID iD
Author: Nicole Grobert
Author: John F. Ryan

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