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Polypyrimidine tract binding protein and poly r(C) binding protein 1 interact with the BAG-1 IRES and stimulate its activity in vitro and in vivo

Record type: Article

The 5'-untranslated region of Bag-1 mRNA contains an internal ribosome entry segment (IRES) and the translation of Bag-1 protein can be initiated by both cap-dependent and cap-independent mechanisms. In general, cellular IRESs require non-canonical trans-acting factors for their activity, however, very few of the proteins that act on cellular IRESs have been identified. Proteins that interact with viral IRESs have also been shown to stimulate the activity of cellular IRESs and therefore the ability of a range of known viral trans-acting factors to stimulate the Bag-1 IRES was tested. Two proteins, poly r(C) binding protein 1 (PCBP1) and polypyrimidine tract binding protein (PTB), were found to increase the activity of the Bag-1 IRES in vitro and in vivo. The regions of the Bag-1 IRES RNA to which they bind have been determined, and it was shown that PCBP1 binds to a short 66 nt section of RNA, whilst PTB interacts with a number of sites over a larger area. The minimum section of the RNA that still retained activity was determined and both PCBP1 and PTB interacted with this region suggesting that these proteins are essential for Bag-1 IRES function.

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Citation

Pickering, Becky M., Mitchell, Sally A., Evans, Joanne R. and Willis, Anne E. (2003) Polypyrimidine tract binding protein and poly r(C) binding protein 1 interact with the BAG-1 IRES and stimulate its activity in vitro and in vivo Nucleic Acids Research, 31, (2), pp. 639-646.

More information

Published date: 2003

Identifiers

Local EPrints ID: 26526
URI: http://eprints.soton.ac.uk/id/eprint/26526
ISSN: 0305-1048
PURE UUID: d24e8f6d-2196-4ff2-aafc-5c3f763f993c

Catalogue record

Date deposited: 19 Apr 2006
Last modified: 17 Jul 2017 16:07

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Contributors

Author: Becky M. Pickering
Author: Sally A. Mitchell
Author: Joanne R. Evans
Author: Anne E. Willis

University divisions


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