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Single cell impedance spectroscopy

Single cell impedance spectroscopy
Single cell impedance spectroscopy
Cellular analysis requires a combination of biophysical and biochemical approaches for counting, manipulation and characterization of biological cells. In recent years, considerable attentions have been paid to single cell analysis based on Lab-On-a-Chip (LOC) technology, which offers the characterization of a large amount of cells one by one [1-3]. Electrical impedance spectroscopy (EIS) provides a high speed, non-invasive and label-free technique for single cell analysis. We have fabricated microfluidic chips with integrated microelectrodes inside the microchannel to perform a differential impedance measurement, as shown in figure 1. Two pairs of parallel facing electrodes define a detection and reference volume. AC excitation voltages at mixed frequencies are applied to the microelectrodes, generating electric field in the channel. As a cell passes by, it modifies the current lines through each of the two detection volumes in turn. A positive and negative peak variation in the measured differential current signal can be observed. Due to differences in the dielectric properties and sizes of various cells, specific information can be obtained from a single cell membrane, cytoplasm or nucleus at distinct frequencies.
Sun, Tao
b2f8e932-a7e6-4fe7-94dd-5c4ce725eacb
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Sun, Tao
b2f8e932-a7e6-4fe7-94dd-5c4ce725eacb
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Sun, Tao and Morgan, Hywel (2008) Single cell impedance spectroscopy. Mini-Symposium on Opto-Microfluidics for Biological Large Scale Integration, The Rank Prize Funds, United Kingdom. 21 - 24 Apr 2008.

Record type: Conference or Workshop Item (Other)

Abstract

Cellular analysis requires a combination of biophysical and biochemical approaches for counting, manipulation and characterization of biological cells. In recent years, considerable attentions have been paid to single cell analysis based on Lab-On-a-Chip (LOC) technology, which offers the characterization of a large amount of cells one by one [1-3]. Electrical impedance spectroscopy (EIS) provides a high speed, non-invasive and label-free technique for single cell analysis. We have fabricated microfluidic chips with integrated microelectrodes inside the microchannel to perform a differential impedance measurement, as shown in figure 1. Two pairs of parallel facing electrodes define a detection and reference volume. AC excitation voltages at mixed frequencies are applied to the microelectrodes, generating electric field in the channel. As a cell passes by, it modifies the current lines through each of the two detection volumes in turn. A positive and negative peak variation in the measured differential current signal can be observed. Due to differences in the dielectric properties and sizes of various cells, specific information can be obtained from a single cell membrane, cytoplasm or nucleus at distinct frequencies.

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More information

Published date: 22 April 2008
Additional Information: Event Dates: 21st-24th April
Venue - Dates: Mini-Symposium on Opto-Microfluidics for Biological Large Scale Integration, The Rank Prize Funds, United Kingdom, 2008-04-21 - 2008-04-24
Organisations: Nanoelectronics and Nanotechnology

Identifiers

Local EPrints ID: 265613
URI: https://eprints.soton.ac.uk/id/eprint/265613
PURE UUID: ecb4bfbd-bbc8-4a71-997c-127e31b32e04
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 24 Apr 2008 20:52
Last modified: 06 Jun 2018 12:45

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Contributors

Author: Tao Sun
Author: Hywel Morgan ORCID iD

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