Karyotyping lymph node biopsies in non-Hodgkin's lymphoma
Karyotyping lymph node biopsies in non-Hodgkin's lymphoma
Culture and transport methods are described that allow chromosomes to be obtained from lymph node biopsies with a high success rate, even when the biopsy is taken at a distant site from the cytogenetic laboratory. Optimal banding techniques for the identification of these chromosomes are given, along with hints on how to correct any problems encountered on initial staining. We also describe how to prepare this material for fluorescence in situ hybridization techniques, both for further delineation of complex chromosomal rearrangements and for interphase analysis in cases in which conventional cytogenetics has not been successful.
chromosomal abnormalities, cytogenetics, fish, ceel culture, chromosome preparations
1592599362
93-108
Ross, Fiona M.
ec0958f8-b992-4e4a-b7e3-c474600390ba
Harrison, Christine J.
52da7673-509c-4b88-b92e-0c021c9c7d3e
2005
Ross, Fiona M.
ec0958f8-b992-4e4a-b7e3-c474600390ba
Harrison, Christine J.
52da7673-509c-4b88-b92e-0c021c9c7d3e
Ross, Fiona M. and Harrison, Christine J.
(2005)
Karyotyping lymph node biopsies in non-Hodgkin's lymphoma.
In,
Illidge, Tim and Johnson, Peter W.M.
(eds.)
Lymphoma: methods and protocols.
(Methods in Molecular Medicine, 115)
Humana Press, .
Record type:
Book Section
Abstract
Culture and transport methods are described that allow chromosomes to be obtained from lymph node biopsies with a high success rate, even when the biopsy is taken at a distant site from the cytogenetic laboratory. Optimal banding techniques for the identification of these chromosomes are given, along with hints on how to correct any problems encountered on initial staining. We also describe how to prepare this material for fluorescence in situ hybridization techniques, both for further delineation of complex chromosomal rearrangements and for interphase analysis in cases in which conventional cytogenetics has not been successful.
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More information
Published date: 2005
Keywords:
chromosomal abnormalities, cytogenetics, fish, ceel culture, chromosome preparations
Identifiers
Local EPrints ID: 26578
URI: http://eprints.soton.ac.uk/id/eprint/26578
ISBN: 1592599362
PURE UUID: ee8a6ba2-20ea-49f3-a4d5-c5aeeed3d5ce
Catalogue record
Date deposited: 20 Apr 2006
Last modified: 22 Jul 2022 20:35
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Contributors
Author:
Fiona M. Ross
Author:
Christine J. Harrison
Editor:
Tim Illidge
Editor:
Peter W.M. Johnson
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