Determining mutational status of immunoglobulin v genes in chronic lymphocytic leukemia: a useful prognostic indicator

Sahota, Surinder S., Babbage, Gavin, Zojer, Niklas, Ottensmeier, Christian H. and Stevenson, Freda K. (2005) Determining mutational status of immunoglobulin v genes in chronic lymphocytic leukemia: a useful prognostic indicator. In, Lymphoma: methods and protocols. (Methods in Molecular Medicine, 115) Humana Press, pp. 129-144.

Abstract

Determining the clonal origins of malignant B-cells will have an impact on disease understanding and management. In this regard, immunoglobulin variable (V) region gene analysis already is having a significant impact in delineating the tumor cell of origin. It can identify, among other features whether such a cell has undergone somatic mutation, which usually occurs within germinal centres. Remarkably, in chronic lymphocytic leukemia (CLL), the mutational status of V genes has allowed researchers to identify two subsets of disease, one originating from an unmutated B-cell with a markedly poorer disease outcome and the other from a mutated B-cell, which associates with long-term survival. The V gene status in CLL thus provides a robust indicator of disease outcome, which is beginning to shape clinical treatment. This chapter describes in detail the methodology for determining V gene usage in CLL, from acquisition of patient sample to generating the V-gene readout.

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Contributors

Author: Freda K. Stevenson

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