Identification of an Arabidopsis inorganic pyrophosphatase capable of being imported into chloroplasts
Identification of an Arabidopsis inorganic pyrophosphatase capable of being imported into chloroplasts
An Arabidopsis cDNA coding for a previously uncharacterized isoform of inorganic pyrophosphatase was isolated. It was used to complement an E. coli mutant, demonstrating that it coded for an active enzyme. MgCl2 was necessary for the protein's activity, whilst NaF inhibited it. The Km for pyrophosphate and the pH optimum of the protein was determined. The gene coding for this protein was expressed in all tissues, and its expression in rosette leaves was induced by incubation on metabolizable sugars. In vitro import experiments demonstrated that the protein could be imported into chloroplasts and localized to the stromal compartment.
inorganic pyrophosphatase, arabidopsis, plastid
101-105
Schulze, Silke
c4fa5204-ec92-416c-a643-f4a921c4a918
Mant, Alexandra
63319e45-deeb-45ad-a30d-e05b42052a0d
Kossmann, Jens
e1e88c5c-345a-4bee-93a1-1a358fc9a474
Lloyd, James R.
c58d7a31-214c-4192-bca1-fc3e62ed93eb
2004
Schulze, Silke
c4fa5204-ec92-416c-a643-f4a921c4a918
Mant, Alexandra
63319e45-deeb-45ad-a30d-e05b42052a0d
Kossmann, Jens
e1e88c5c-345a-4bee-93a1-1a358fc9a474
Lloyd, James R.
c58d7a31-214c-4192-bca1-fc3e62ed93eb
Schulze, Silke, Mant, Alexandra, Kossmann, Jens and Lloyd, James R.
(2004)
Identification of an Arabidopsis inorganic pyrophosphatase capable of being imported into chloroplasts.
FEBS Letters, 565 (1-3), .
(doi:10.1016/j.febslet.2004.03.080).
Abstract
An Arabidopsis cDNA coding for a previously uncharacterized isoform of inorganic pyrophosphatase was isolated. It was used to complement an E. coli mutant, demonstrating that it coded for an active enzyme. MgCl2 was necessary for the protein's activity, whilst NaF inhibited it. The Km for pyrophosphate and the pH optimum of the protein was determined. The gene coding for this protein was expressed in all tissues, and its expression in rosette leaves was induced by incubation on metabolizable sugars. In vitro import experiments demonstrated that the protein could be imported into chloroplasts and localized to the stromal compartment.
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Published date: 2004
Keywords:
inorganic pyrophosphatase, arabidopsis, plastid
Identifiers
Local EPrints ID: 26597
URI: http://eprints.soton.ac.uk/id/eprint/26597
PURE UUID: 6ddee6d0-ee46-4187-bd6a-5976d948579a
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Date deposited: 20 Apr 2006
Last modified: 16 Mar 2024 03:40
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Contributors
Author:
Silke Schulze
Author:
Alexandra Mant
Author:
Jens Kossmann
Author:
James R. Lloyd
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