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Conservation and variation in human and common chimpanzee CD94 and NKG2 genes

Conservation and variation in human and common chimpanzee CD94 and NKG2 genes
Conservation and variation in human and common chimpanzee CD94 and NKG2 genes
To assess polymorphism and variation in human and chimpanzee NK complex genes, we determined the coding-region sequences for CD94 and NKG2A, C, D, E, and F from several human (Homo sapiens) donors and common chimpanzees (Pan troglodytes). CD94 is highly conserved, while the NKG2 genes exhibit some polymorphism. For all the genes, alternative mRNA splicing variants were frequent among the clones obtained by RT-PCR.
Alternative splicing acts similarly in human and chimpanzee to produce the CD94B variant from the CD94 gene and the NKG2B variant from the NKG2A gene. Whereas single chimpanzee orthologs for CD94, NKG2A, NKG2E, and NKG2F were identified, two chimpanzee paralogs of the human NKG2C gene were defined. The chimpanzee Pt-NKG2CI gene encodes a protein similar to human NKG2C, whereas in the chimpanzee Pt-NKG2CII gene the translation frame changes near the beginning of the carbohydrate recognition domain, causing premature termination.
Analysis of a panel of chimpanzee NK cell clones showed that Pt-NKG2CI and Pt-NKG2CII are independently and clonally expressed. Pt-NKG2CI and Pt-NKG2CII are equally diverged from human NKG2C, indicating that they arose by gene duplication subsequent to the divergence of chimpanzee and human ancestors. Genomic DNA from 80 individuals representing six primate species were typed for the presence of CD94 and NKG2. Each species gave distinctive typing patterns, with NKG2A and CD94 being most conserved. Seven different NK complex genotypes within the panel of 48 common chimpanzees were due to differences in Pt-NKG2C and Pt-NKG2D genes.
0022-1767
240-252
Shum, Benny P.
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Flodin, Laura R.
e4e8a481-1e6a-4767-b538-62c75614bf97
Muir, David G.
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Rajalingam, Raja
cfaf4da4-5bac-42ab-a235-8fc700b65987
Khakoo, Salim I.
6c16d2f5-ae80-4d9b-9100-6bfb34ad0273
Cleland, Sophia
1007eb88-2d29-41d0-8cd7-6a2860178f7a
Guethlein, Lisabeth A.
ca54bf0d-dbe5-46f3-aa15-9b7ef7ef6bb7
Uhrberg, Markus
3e577d7a-9513-48de-8dc9-313a547e2821
Parham, Peter
85caaa6b-edd8-4239-b69b-b24043d52f9e
Shum, Benny P.
b487c075-3263-4489-a5dc-8aa92fab4970
Flodin, Laura R.
e4e8a481-1e6a-4767-b538-62c75614bf97
Muir, David G.
64257a0c-1c56-4cf7-b184-90bc03f50768
Rajalingam, Raja
cfaf4da4-5bac-42ab-a235-8fc700b65987
Khakoo, Salim I.
6c16d2f5-ae80-4d9b-9100-6bfb34ad0273
Cleland, Sophia
1007eb88-2d29-41d0-8cd7-6a2860178f7a
Guethlein, Lisabeth A.
ca54bf0d-dbe5-46f3-aa15-9b7ef7ef6bb7
Uhrberg, Markus
3e577d7a-9513-48de-8dc9-313a547e2821
Parham, Peter
85caaa6b-edd8-4239-b69b-b24043d52f9e

Shum, Benny P., Flodin, Laura R., Muir, David G., Rajalingam, Raja, Khakoo, Salim I., Cleland, Sophia, Guethlein, Lisabeth A., Uhrberg, Markus and Parham, Peter (2002) Conservation and variation in human and common chimpanzee CD94 and NKG2 genes. The Journal of Immunology, 168 (1), 240-252.

Record type: Article

Abstract

To assess polymorphism and variation in human and chimpanzee NK complex genes, we determined the coding-region sequences for CD94 and NKG2A, C, D, E, and F from several human (Homo sapiens) donors and common chimpanzees (Pan troglodytes). CD94 is highly conserved, while the NKG2 genes exhibit some polymorphism. For all the genes, alternative mRNA splicing variants were frequent among the clones obtained by RT-PCR.
Alternative splicing acts similarly in human and chimpanzee to produce the CD94B variant from the CD94 gene and the NKG2B variant from the NKG2A gene. Whereas single chimpanzee orthologs for CD94, NKG2A, NKG2E, and NKG2F were identified, two chimpanzee paralogs of the human NKG2C gene were defined. The chimpanzee Pt-NKG2CI gene encodes a protein similar to human NKG2C, whereas in the chimpanzee Pt-NKG2CII gene the translation frame changes near the beginning of the carbohydrate recognition domain, causing premature termination.
Analysis of a panel of chimpanzee NK cell clones showed that Pt-NKG2CI and Pt-NKG2CII are independently and clonally expressed. Pt-NKG2CI and Pt-NKG2CII are equally diverged from human NKG2C, indicating that they arose by gene duplication subsequent to the divergence of chimpanzee and human ancestors. Genomic DNA from 80 individuals representing six primate species were typed for the presence of CD94 and NKG2. Each species gave distinctive typing patterns, with NKG2A and CD94 being most conserved. Seven different NK complex genotypes within the panel of 48 common chimpanzees were due to differences in Pt-NKG2C and Pt-NKG2D genes.

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Published date: 2002

Identifiers

Local EPrints ID: 26606
URI: http://eprints.soton.ac.uk/id/eprint/26606
ISSN: 0022-1767
PURE UUID: 23aa9baf-a78a-4acd-abdf-741b040f907e

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Date deposited: 20 Apr 2006
Last modified: 15 Jul 2019 19:13

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Contributors

Author: Benny P. Shum
Author: Laura R. Flodin
Author: David G. Muir
Author: Raja Rajalingam
Author: Salim I. Khakoo
Author: Sophia Cleland
Author: Lisabeth A. Guethlein
Author: Markus Uhrberg
Author: Peter Parham

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