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The characterisation of the lymphoma cell line U937, using comparative genomic hybridisation and multi-plex FISH

Strefford, J.C., Foot, N.J., Chaplin, T., Neat, M.J., Oliver, R.T.D., Young, B.D. and Jones, L.K. (2001) The characterisation of the lymphoma cell line U937, using comparative genomic hybridisation and multi-plex FISH Cytogenetics and Cell Genetics, 94, (1-2), pp. 9-14. (doi:10.1159/000048774).

Record type: Article

Abstract

The cell line U937, which has been used extensively for studies of myeloid differentiation, bears the t(10;11)(p13;q14) translocation which results in a fusion between the MLLT10 (myeloid/lymphoid or mixed-lineage leukemia [trithorax, Drosophila, homolog]; translocated to 10; alias AF10) gene and the Ap-3-like clathrin assembly protein, PICALM (Clathrin assembly lymphoid myeloid leukaemia). Apart from this translocation, very little is known about the other genetic alterations in this cell line that may represent significant events in disease progression. In this study, conventional G-banding, CGH and M-FISH have been used to characterise fully all of the cytogenetic alterations present in the U937 cell line. M-FISH analysis confirmed the presence of the t(10;11) and an apparently normal copy of both chromosomes 10 and 11. A t(1;5) translocation was observed as well as several unbalanced rearrangements. CGH detected amplifications resulting from duplications of 2q, 6p and 13q. These changes could result in fusion gene products involved in carcinogenesis or the positions of putative oncogenes and tumour suppressor genes. A good correlation between conventional G-banding, CGH and M-FISH was observed.

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Published date: 2001

Identifiers

Local EPrints ID: 26624
URI: http://eprints.soton.ac.uk/id/eprint/26624
PURE UUID: 9cce598b-278d-429b-ae03-55ee604d9729
ORCID for J.C. Strefford: ORCID iD orcid.org/0000-0002-0972-2881

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Date deposited: 20 Apr 2006
Last modified: 17 Jul 2017 16:06

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Author: J.C. Strefford ORCID iD
Author: N.J. Foot
Author: T. Chaplin
Author: M.J. Neat
Author: R.T.D. Oliver
Author: B.D. Young
Author: L.K. Jones

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