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The use of multicolor fluorescence technologies in the characterization of prostate carcinoma cell lines: a comparison of multiplex fluorescence in situ hybridization and spectral karyotyping data

Strefford, Jon C., Lillington, Debra M., Young, Bryan D. and Oliver, R.T.D. (2001) The use of multicolor fluorescence technologies in the characterization of prostate carcinoma cell lines: a comparison of multiplex fluorescence in situ hybridization and spectral karyotyping data Cancer Genetics and Cytogenetics, 124, (2), pp. 112-121. (doi:10.1016/S0165-4608(00)00339-3).

Record type: Article

Abstract

Recent studies have identified several chromosome regions that are altered in primary prostate cancer and prostatic carcinoma cell lines. These targeted regions may harbor genes involved in tumor suppression. We used multiplex fluorescence in situ hybridization (M-FISH) to screen for genetic rearrangements in four prostate cancer cell lines, LNCaP, LNCaP.FCG, DU145, and PC3, and compared our results with those recently obtained using spectral karyotyping (SKY). A number of differences was noted between abnormalities characterized by SKY and M-FISH, suggesting variation in karyotype evolution and characterization by these two methodologies. M-FISH analysis showed that hormone-resistant cell lines (DU145 and PC3) contained many genetic alterations (?15 per cell), suggesting high levels of genetic instability in hormone-refractory prostate cancer. Most chromosome regions previously implicated in prostate cancer were altered in one or more of these cell lines. Several specific chromosome aberrations were also detected, including a del(4)(p14) and a del(6)(q21) in the hormone-insensitive cell lines, a t(1;15)(p?;q?) in LNCaP, LNCaP, and PC3, and a i(5p) in LNCaP.FCG, DU145, and PC3. These clonal chromosome abnormalities may pinpoint gene loci associated with prostate tumourigenesis, cancer progression, and hormone sensitivity.

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Published date: 2001

Identifiers

Local EPrints ID: 26625
URI: http://eprints.soton.ac.uk/id/eprint/26625
ISSN: 0165-4608
PURE UUID: 3d0b9464-cf33-40c6-a0bc-67ac35fb9bad
ORCID for Jon C. Strefford: ORCID iD orcid.org/0000-0002-0972-2881

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Date deposited: 20 Apr 2006
Last modified: 17 Jul 2017 16:06

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Contributors

Author: Debra M. Lillington
Author: Bryan D. Young
Author: R.T.D. Oliver

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