The University of Southampton
University of Southampton Institutional Repository

Novel chromosome findings in bladder cancer cell lines detected with multiplex fluorescence in situ hybridization

Novel chromosome findings in bladder cancer cell lines detected with multiplex fluorescence in situ hybridization
Novel chromosome findings in bladder cancer cell lines detected with multiplex fluorescence in situ hybridization
Bladder cancer is a common neoplasm worldwide, consisting mainly of transitional cell carcinomas, while squamous, adenocarcinoma, and sarcomatoid bladder cancers account for the remaining cases. In the present study, multiplex fluorescence in situ hybridization (M-FISH) has been used to characterize chromosome rearrangements in eight transitional and one squamous cell carcinoma cell line, RT112, of UMUC-3, 5637, CAT(wil), FGEN, EJ28, J82, 253J, and SCaBER. Alterations of chromosome 9 are the most frequent cytogenetic and molecular findings in transitional cell carcinomas of all grades and stages, while changes of chromosomes 3, 4, 8, 9, 11, 14, and 17 are also frequently observed. In the present study, alterations previously described, including del(8)(p10), del(9)(p10), del(17)(p10), and overrepresentation of chromosome 20, as well as several novel findings, were observed. These novel findings were a del(15)(q15) and isochromosome 14q, both occurring in three of nine cell lines examined. These abnormalities may reflect changes in bladder tumor biology. M-FISH represents an effective preliminary screening tool for the characterization of complex tumor karyotypes.
0165-4608
139-146
Strefford, Jon C.
3782b392-f080-42bf-bdca-8aa5d6ca532f
Lillington, Debra M.
a014d08c-943d-4426-8872-dc0db9adb167
Steggall, Martin
c22c6d3f-9e68-4f48-85f1-2ac82d92ec9b
Lane, Tim M.
91a527f4-1b81-47a6-bd89-118249142bc2
Nouri, A.M.E
776d5e38-75aa-43bc-bf41-2edea427c030
Young, Bryan D.
d6d3ff53-6b73-42e7-8d41-19d1f16c58d2
Oliver, R.T.D.
72fd34ac-3d25-469b-8515-05d0bc0c7e25
Strefford, Jon C.
3782b392-f080-42bf-bdca-8aa5d6ca532f
Lillington, Debra M.
a014d08c-943d-4426-8872-dc0db9adb167
Steggall, Martin
c22c6d3f-9e68-4f48-85f1-2ac82d92ec9b
Lane, Tim M.
91a527f4-1b81-47a6-bd89-118249142bc2
Nouri, A.M.E
776d5e38-75aa-43bc-bf41-2edea427c030
Young, Bryan D.
d6d3ff53-6b73-42e7-8d41-19d1f16c58d2
Oliver, R.T.D.
72fd34ac-3d25-469b-8515-05d0bc0c7e25

Strefford, Jon C., Lillington, Debra M., Steggall, Martin, Lane, Tim M., Nouri, A.M.E, Young, Bryan D. and Oliver, R.T.D. (2002) Novel chromosome findings in bladder cancer cell lines detected with multiplex fluorescence in situ hybridization. Cancer Genetics and Cytogenetics, 135 (2), 139-146. (doi:10.1016/S0165-4608(01)00648-3).

Record type: Article

Abstract

Bladder cancer is a common neoplasm worldwide, consisting mainly of transitional cell carcinomas, while squamous, adenocarcinoma, and sarcomatoid bladder cancers account for the remaining cases. In the present study, multiplex fluorescence in situ hybridization (M-FISH) has been used to characterize chromosome rearrangements in eight transitional and one squamous cell carcinoma cell line, RT112, of UMUC-3, 5637, CAT(wil), FGEN, EJ28, J82, 253J, and SCaBER. Alterations of chromosome 9 are the most frequent cytogenetic and molecular findings in transitional cell carcinomas of all grades and stages, while changes of chromosomes 3, 4, 8, 9, 11, 14, and 17 are also frequently observed. In the present study, alterations previously described, including del(8)(p10), del(9)(p10), del(17)(p10), and overrepresentation of chromosome 20, as well as several novel findings, were observed. These novel findings were a del(15)(q15) and isochromosome 14q, both occurring in three of nine cell lines examined. These abnormalities may reflect changes in bladder tumor biology. M-FISH represents an effective preliminary screening tool for the characterization of complex tumor karyotypes.

Full text not available from this repository.

More information

Published date: 2002

Identifiers

Local EPrints ID: 26626
URI: http://eprints.soton.ac.uk/id/eprint/26626
ISSN: 0165-4608
PURE UUID: d96c7d9e-8178-48c9-b85b-da3e81dd40e9
ORCID for Jon C. Strefford: ORCID iD orcid.org/0000-0002-0972-2881

Catalogue record

Date deposited: 21 Apr 2006
Last modified: 03 Dec 2019 01:48

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×