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A minimal Bcl-x promoter is activated by Brn-3a and repressed by p53

A minimal Bcl-x promoter is activated by Brn-3a and repressed by p53
A minimal Bcl-x promoter is activated by Brn-3a and repressed by p53
The Brn-3a transcription factor stimulates the expression of the anti-apoptotic Bcl-2 and Bcl-x proteins and protects neuronal cells from apoptosis. Here we show that a minimal Bcl-x promoter is activated by Brn-3a and that this stimulation is prevented by the pro-apoptotic p53 protein. Both these effects are mediated via Bcl-x promoter sequences, which are indistinguishable from those required for minimal basal promoter activity. A newly described upstream Bcl-x promoter is also activated by Brn-3a with this activation being prevented by p53. Hence, Brn-3a-mediated activation of two distinct Bcl-x promoters and of the Bcl-2 promoter is blocked by p53 whereas this is not observed for Brn-3a activated promoters derived from genes not involved in inhibiting apoptosis. p53 therefore appears to specifically target the activation by Brn-3a of promoters derived from genes with an anti-apoptotic effect and this may be involved in the pro-apoptotic activity of p53.
0305-1048
4530-4540
Sugars, Katherine L.
fe10ba7c-94d8-4166-9192-1c5d46dc767b
Budhram-Mahadeo, Vishwanie
ae58e45d-4480-476d-bd66-357c34bfb90e
Packham, Graham
fdabe56f-2c58-469c-aadf-38878f233394
Latchman, David S.
71e9db7c-9075-4b49-afac-6413085378db
Sugars, Katherine L.
fe10ba7c-94d8-4166-9192-1c5d46dc767b
Budhram-Mahadeo, Vishwanie
ae58e45d-4480-476d-bd66-357c34bfb90e
Packham, Graham
fdabe56f-2c58-469c-aadf-38878f233394
Latchman, David S.
71e9db7c-9075-4b49-afac-6413085378db

Sugars, Katherine L., Budhram-Mahadeo, Vishwanie, Packham, Graham and Latchman, David S. (2001) A minimal Bcl-x promoter is activated by Brn-3a and repressed by p53. Nucleic Acids Research, 29 (22), 4530-4540. (doi:10.1093/nar/29.22.4530).

Record type: Article

Abstract

The Brn-3a transcription factor stimulates the expression of the anti-apoptotic Bcl-2 and Bcl-x proteins and protects neuronal cells from apoptosis. Here we show that a minimal Bcl-x promoter is activated by Brn-3a and that this stimulation is prevented by the pro-apoptotic p53 protein. Both these effects are mediated via Bcl-x promoter sequences, which are indistinguishable from those required for minimal basal promoter activity. A newly described upstream Bcl-x promoter is also activated by Brn-3a with this activation being prevented by p53. Hence, Brn-3a-mediated activation of two distinct Bcl-x promoters and of the Bcl-2 promoter is blocked by p53 whereas this is not observed for Brn-3a activated promoters derived from genes not involved in inhibiting apoptosis. p53 therefore appears to specifically target the activation by Brn-3a of promoters derived from genes with an anti-apoptotic effect and this may be involved in the pro-apoptotic activity of p53.

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Published date: 2001

Identifiers

Local EPrints ID: 26630
URI: http://eprints.soton.ac.uk/id/eprint/26630
ISSN: 0305-1048
PURE UUID: 32848078-bad7-48ab-9011-9d35119b0e2a
ORCID for Graham Packham: ORCID iD orcid.org/0000-0002-9232-5691

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Date deposited: 24 Apr 2006
Last modified: 16 Mar 2024 03:14

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Contributors

Author: Katherine L. Sugars
Author: Vishwanie Budhram-Mahadeo
Author: Graham Packham ORCID iD
Author: David S. Latchman

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