Overexpression of genes on 16q associated with cisplatin resistance of testicular germ cell tumor cell lines
Overexpression of genes on 16q associated with cisplatin resistance of testicular germ cell tumor cell lines
Testicular germ-cell tumors (TGCTs) show exquisite sensitivity to cisplatin-based chemotherapy, and therefore this is considered a good model system for studying the mechanism of chemotherapy resistance. Although the genetic alterations related to TGCT have been well studied, little is known about the genetic basis of chemotherapy resistance, which occurs in a small proportion of TGCTs. In this study, we investigated genomic and expression differences between three cisplatin-sensitive and their paired cisplatin-resistant lines using combined whole-genome screen approaches. Comparative genomic hybridization (CGH) analysis on chromosomes revealed genetic differences between the resistant and parent cell lines in each pair, but did not show any consistent chromosome changes in all three lines. Microarray CGH analysis generated some additional information of DNA copy number gains and losses including some important oncogenes, tumor-suppressor genes, and drug-resistance-related genes. However, no consistent genomic region changes were found in the three cell lines. Interestingly, when comparative expressed sequence hybridization, a technique for gene expression profiling along chromosomes, was applied, we discovered a consistently overexpressed chromosomal region in all three resistant lines compared with their parent lines. The minimum overlapping chromosomal region is at 16q22-23. Further definition of genes in this chromosomal region will aid our understanding of the mechanism of cisplatin resistance and may offer novel therapeutic targets.
211-216
Wilson, Charlotte
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Yang, Jinshu
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Strefford, Jon C.
3782b392-f080-42bf-bdca-8aa5d6ca532f
Summersgill, Brenda
cd1829c5-f735-46c1-a1c6-e57dd507456e
Young, Bryan D.
d6d3ff53-6b73-42e7-8d41-19d1f16c58d2
Shipley, Janet
c4bd3760-d826-42ba-8321-ce78582034ac
Oliver, Tim
13287ba1-310d-424f-b5a5-fe27dab03664
Lu, Yong-Jie
e33687b0-c0b5-4820-998a-dd0b1540c4eb
2005
Wilson, Charlotte
04387b41-3e60-496b-90d4-fd02e3efa3c7
Yang, Jinshu
ca5f86ad-5fe9-4c3f-af66-222b0121a342
Strefford, Jon C.
3782b392-f080-42bf-bdca-8aa5d6ca532f
Summersgill, Brenda
cd1829c5-f735-46c1-a1c6-e57dd507456e
Young, Bryan D.
d6d3ff53-6b73-42e7-8d41-19d1f16c58d2
Shipley, Janet
c4bd3760-d826-42ba-8321-ce78582034ac
Oliver, Tim
13287ba1-310d-424f-b5a5-fe27dab03664
Lu, Yong-Jie
e33687b0-c0b5-4820-998a-dd0b1540c4eb
Wilson, Charlotte, Yang, Jinshu, Strefford, Jon C., Summersgill, Brenda, Young, Bryan D., Shipley, Janet, Oliver, Tim and Lu, Yong-Jie
(2005)
Overexpression of genes on 16q associated with cisplatin resistance of testicular germ cell tumor cell lines.
Genes, Chromosomes and Cancer, 43 (2), .
(doi:10.1002/gcc.20173).
Abstract
Testicular germ-cell tumors (TGCTs) show exquisite sensitivity to cisplatin-based chemotherapy, and therefore this is considered a good model system for studying the mechanism of chemotherapy resistance. Although the genetic alterations related to TGCT have been well studied, little is known about the genetic basis of chemotherapy resistance, which occurs in a small proportion of TGCTs. In this study, we investigated genomic and expression differences between three cisplatin-sensitive and their paired cisplatin-resistant lines using combined whole-genome screen approaches. Comparative genomic hybridization (CGH) analysis on chromosomes revealed genetic differences between the resistant and parent cell lines in each pair, but did not show any consistent chromosome changes in all three lines. Microarray CGH analysis generated some additional information of DNA copy number gains and losses including some important oncogenes, tumor-suppressor genes, and drug-resistance-related genes. However, no consistent genomic region changes were found in the three cell lines. Interestingly, when comparative expressed sequence hybridization, a technique for gene expression profiling along chromosomes, was applied, we discovered a consistently overexpressed chromosomal region in all three resistant lines compared with their parent lines. The minimum overlapping chromosomal region is at 16q22-23. Further definition of genes in this chromosomal region will aid our understanding of the mechanism of cisplatin resistance and may offer novel therapeutic targets.
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Published date: 2005
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Brief Communication
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Local EPrints ID: 26665
URI: http://eprints.soton.ac.uk/id/eprint/26665
ISSN: 1045-2257
PURE UUID: 4916c42a-3f3f-4814-ae3c-219666870c18
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Date deposited: 10 Apr 2006
Last modified: 16 Mar 2024 03:40
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Author:
Charlotte Wilson
Author:
Jinshu Yang
Author:
Brenda Summersgill
Author:
Bryan D. Young
Author:
Janet Shipley
Author:
Tim Oliver
Author:
Yong-Jie Lu
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