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Distinct Albino3-dependent and -independent pathways for thylakoid membrane protein insertion

Record type: Article

The homologous proteins Oxa1, YidC, and Alb3 mediate the insertion of membrane proteins in mitochondria, bacteria, and chloroplast thylakoids, respectively. Depletion of YidC in Escherichia coli affects the integration of every membrane protein studied, and Alb3 has been shown previously to be required for the insertion of a signal recognition particle (SRP)-dependent protein, Lhcb1, in thylakoids. In this study we have analyzed the "global" role of Alb3 in the insertion of thylakoid membrane proteins. We show that insertion of two chlorophyll-binding proteins, Lhcb4.1 and Lhcb5, is almost totally blocked by preincubation of thylakoids with anti-Alb3 antibodies, indicating a requirement for Alb3 in the insertion pathway. Insertion of the related PsbS protein, on the other hand, is unaffected by Alb3 antibodies, and insertion of a group of SRP-independent, signal peptide-bearing proteins, PsbX, PsbW, and PsbY, is likewise completely unaffected. Proteinase K is furthermore able to completely degrade Alb3, but this treatment does not affect the insertion of these proteins. Among the thylakoid proteins studied here, Alb3 requirement correlates strictly with a requirement for stromal factors and nucleoside triphosphates. However, the majority of proteins tested do not require Alb3 or any other known form of translocation apparatus.

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Citation

Woolhead, Cheryl A., Thompson, Simon J., Moore, Misty, Tissier, Christophe, Mant, Alexandra, Rodger, Alison, Henry, Ralph and Robinson, Colin (2001) Distinct Albino3-dependent and -independent pathways for thylakoid membrane protein insertion The Journal of Biological Chemistry, 276, (44), pp. 40841-40846. (doi:10.1074/jbc.M106523200). (PMID:11524428).

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Published date: 2 November 2001

Identifiers

Local EPrints ID: 26668
URI: http://eprints.soton.ac.uk/id/eprint/26668
ISSN: 0021-9258
PURE UUID: d3ba934c-2356-4036-bbca-7ece4b7eede7

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Date deposited: 24 Mar 2010
Last modified: 17 Jul 2017 16:06

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Contributors

Author: Cheryl A. Woolhead
Author: Simon J. Thompson
Author: Misty Moore
Author: Christophe Tissier
Author: Alexandra Mant
Author: Alison Rodger
Author: Ralph Henry
Author: Colin Robinson

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